Literature DB >> 12944023

Detection of dihydrofolate reductase genes by PCR and RFLP.

Margarita M Navia1, Joaquim Ruiz, Javier Sanchez-Cespedes, Jordi Vila.   

Abstract

The presence of plasmid-encoded trimethoprim resistant dfr genes is the most common mechanism responsible for the acquisition of trimethoprim resistance. The usual method to detect the presence of these genes is hybridization with specific probes. We describe an alternative, faster and easier method, based on PCR amplification and RFLP analysis, to discriminate up to sixteen different dfr genes.

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Year:  2003        PMID： 12944023     DOI： 10.1016/s0732-8893(03)00062-2

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  12 in total

1.  Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru.

Authors:  Anicia M Medina; Fulton P Rivera; Maria J Pons; Maribel Riveros; Cláudia Gomes; María Bernal; Rina Meza; Ryan C Maves; Luis Huicho; Elsa Chea-Woo; Claudio F Lanata; Ana I Gil; Theresa J Ochoa; Joaquim Ruiz
Journal:  Trans R Soc Trop Med Hyg       Date:  2015-08       Impact factor: 2.184

2.  Profiling Virulence and Antimicrobial Resistance Markers of Enterovirulent Escherichia Coli from Fecal Isolates of Adult Patients with Enteric Infections in West Cameroon.

Authors:  Wiliane J T Marbou; Priyanka Jain; Sriparna Samajpati; Gourab Halder; Asish K Mukhopadhyay; Shanta Dutta; Victor Kuete
Journal:  Osong Public Health Res Perspect       Date:  2020-08

3.  Mechanisms of resistance in multiple-antibiotic-resistant Escherichia coli strains of human, animal, and food origins.

Authors:  Yolanda Sáenz; Laura Briñas; Elena Domínguez; Joaquim Ruiz; Myriam Zarazaga; Jordi Vila; Carmen Torres
Journal:  Antimicrob Agents Chemother       Date:  2004-10       Impact factor: 5.191

4.  Mechanism of resistance to several antimicrobial agents in Salmonella Clinical isolates causing traveler's diarrhea.

Authors:  Roberto Cabrera; Joaquím Ruiz; Francesc Marco; Inés Oliveira; Margarita Arroyo; Ana Aladueña; Miguel A Usera; M Teresa Jiménez De Anta; Joaquím Gascón; Jordi Vila
Journal:  Antimicrob Agents Chemother       Date:  2004-10       Impact factor: 5.191

5.  Molecular mechanisms of antibiotic resistance in diarrhoeagenic Escherichia coli isolated from children.

Authors:  Susan Mosquito; Joaquim Ruiz; María J Pons; David Durand; Francesca Barletta; Theresa J Ochoa
Journal:  Int J Antimicrob Agents       Date:  2012-10-15       Impact factor: 5.283

6.  Antimicrobial susceptibility and mechanisms of resistance in Shigella and Salmonella isolates from children under five years of age with diarrhea in rural Mozambique.

Authors:  Inácio Mandomando; Dinis Jaintilal; Maria J Pons; Xavier Vallès; Mateu Espasa; Laura Mensa; Betuel Sigaúque; Sergi Sanz; Jahit Sacarlal; Eusébio Macete; Fátima Abacassamo; Pedro L Alonso; Joaquim Ruiz
Journal:  Antimicrob Agents Chemother       Date:  2009-03-30       Impact factor: 5.191

7.  Distribution of Integrons and Phylogenetic Groups among Enteropathogenic Escherichia coli Isolates from Children <5 Years of Age in Delhi, India.

Authors:  Taru Singh; Shukla Das; V G Ramachandran; Sayim Wani; Dheeraj Shah; Khan A Maroof; Aditi Sharma
Journal:  Front Microbiol       Date:  2017-04-10       Impact factor: 5.640

8.  Antibiotic Resistant and Biofilm-Associated Escherichia coli Isolates from Diarrheic and Healthy Dogs.

Authors:  Lívia Karahutová; René Mandelík; Dobroslava Bujňáková
Journal:  Microorganisms       Date:  2021-06-19

9.  Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element.

Authors:  Japheth A Opintan; Mercy J Newman; Owusu Agyemang Nsiah-Poodoh; Iruka N Okeke
Journal:  J Antimicrob Chemother       Date:  2008-08-27       Impact factor: 5.790

10.  Antibiotic resistance in bacterial isolates from freshwater samples in Fildes Peninsula, King George Island, Antarctica.

Authors:  Daniela Jara; Helia Bello-Toledo; Mariana Domínguez; Camila Cigarroa; Paulina Fernández; Luis Vergara; Mario Quezada-Aguiluz; Andrés Opazo-Capurro; Celia A Lima; Gerardo González-Rocha
Journal:  Sci Rep       Date:  2020-02-21       Impact factor: 4.379
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