BACKGROUND AND METHOD: We performed differential-display polymerase chain reaction to find up-regulated sequences in primary human gastric cancers, and cloned one up-regulated sequence, which was expressed in all the gastric cancer cells that we examined. The cloned sequence was identified as cytoskeletal-associated protein 2 (CKAP2). We also cloned a shorter splice variant, CKAP2-s. The CKAP2 or CKAP2-s protein in HeLa cells was localized to microtubule organizing centers (MTOC) and microtubules. This co-localization pattern was disrupted by nocodazole, a microtubule-destabilizing agent. RESULTS: These observations suggested that CKAP2 might be associated with microtubule networks. CKAP2 protein was detected in neither normal GI tract nor normal gastric mucosa. However, both CKAP2 and CKAP2-s mRNAs were up-regulated in 55% (23 out of 42 samples) of primary human gastric cancers by reverse transcriptase-polymerase chain reaction (RT-PCR). Moreover, CKAP2 proteins were detected in immunohistochemical staining in all the gastric cancer samples that we examined. CKAP2 protein-expressing cells were also found in gastric adenomas. The average number of CKAP2 protein-positive cells in adenocarcinomas was 48.8%, which was significantly higher than the number in tubular adenomas, 9.1%. CONCLUSION: When these points were taken together, we concluded that CKAP2 is up-regulated in primary human gastric adenocarcinomas at high frequency and might be useful for diagnosing and discriminating adenocarcinomas from tubular adenomas of the stomach.
BACKGROUND AND METHOD: We performed differential-display polymerase chain reaction to find up-regulated sequences in primary humangastric cancers, and cloned one up-regulated sequence, which was expressed in all the gastric cancer cells that we examined. The cloned sequence was identified as cytoskeletal-associated protein 2 (CKAP2). We also cloned a shorter splice variant, CKAP2-s. The CKAP2 or CKAP2-s protein in HeLa cells was localized to microtubule organizing centers (MTOC) and microtubules. This co-localization pattern was disrupted by nocodazole, a microtubule-destabilizing agent. RESULTS: These observations suggested that CKAP2 might be associated with microtubule networks. CKAP2 protein was detected in neither normal GI tract nor normal gastric mucosa. However, both CKAP2 and CKAP2-s mRNAs were up-regulated in 55% (23 out of 42 samples) of primary humangastric cancers by reverse transcriptase-polymerase chain reaction (RT-PCR). Moreover, CKAP2 proteins were detected in immunohistochemical staining in all the gastric cancer samples that we examined. CKAP2 protein-expressing cells were also found in gastric adenomas. The average number of CKAP2 protein-positive cells in adenocarcinomas was 48.8%, which was significantly higher than the number in tubular adenomas, 9.1%. CONCLUSION: When these points were taken together, we concluded that CKAP2 is up-regulated in primary humangastric adenocarcinomas at high frequency and might be useful for diagnosing and discriminating adenocarcinomas from tubular adenomas of the stomach.
Authors: L Maouche-Chrétien; N Deleu; C Badoual; P Fraissignes; R Berger; P Gaulard; P H Roméo; K Leroy-Viard Journal: Oncogene Date: 1998-09-10 Impact factor: 9.867
Authors: Kai Lee Yap; Kazuma Kiyotani; Kenji Tamura; Tatjana Antic; Miran Jang; Magdeline Montoya; Alexa Campanile; Poh Yin Yew; Cory Ganshert; Tomoaki Fujioka; Gary D Steinberg; Peter H O'Donnell; Yusuke Nakamura Journal: Clin Cancer Res Date: 2014-10-14 Impact factor: 12.531
Authors: Chanelle M Case; Dan L Sackett; Danny Wangsa; Tatiana Karpova; James G McNally; Thomas Ried; Jordi Camps Journal: PLoS One Date: 2013-05-30 Impact factor: 3.240