| Literature DB >> 12941923 |
Audrey Brussel1, Pierre Sonigo.
Abstract
A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type 1 (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.Entities:
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Year: 2003 PMID: 12941923 PMCID: PMC224570 DOI: 10.1128/jvi.77.18.10119-10124.2003
Source DB: PubMed Journal: J Virol ISSN: 0022-538X Impact factor: 5.103