Association between HPV 16/18 infection and expression of P53 protein in laryngeal papillomas.

To understand the relationship between expression of P53 protein and HPV16/18 infection in laryngeal papillomas, PCR and immunohistochemical techniques were used to examine the paraffin-embedded tissue samples of laryngeal papillomas from 35 subjects. HPV 16/18-DNA was found in 24 cases of laryngeal papillomas (68.8%). Overexpression of P53 protein was detected in 19 cases (54.3%). Both HPV16/18-DNA and overexpression of P53 protein were demonstrated in 12 cases of laryngeal papillomas (34.3%). Our results suggest that HPV16/18 infection and P53 gene mutation are associated with pathogenesis of laryngeal papillomas. The relation between HPV infection and P53 mutation in tissues of laryngeal papillomas remains to be clarified.