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Literature DB >> 12935424

Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.

Renli Zhang¹, Zhongmin Guo, Jiahai Lu, Jinxiu Meng, Canquan Zhou, Ximei Zhan, Bing Huang, Xinbing Yu, Min Huang, Xinghua Pan, Wenhua Ling, Xigu Chen, Zhuoyue Wan, Huanying Zheng, Xinge Yan, Yifei Wang, Yanchao Ran, Xinjian Liu, Junxin Ma, Chengyu Wang, Biliang Zhang.

Abstract

OBJECTIVE: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases.
METHODS: Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells.
RESULTS: Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect.
CONCLUSION: siRNA may be effective in inhibiting SARS-associated coronavirus replication.

Entities: Disease

Mesh：

Substances：
RNA, Small Interfering

Year: 2003 PMID： 12935424

Source DB: PubMed Journal: Chin Med J (Engl) ISSN： 0366-6999 Impact factor: 2.628

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Cited

16 in total

1. Application of siRNA against SARS in the rhesus macaque model.

Authors: Qingquan Tang; Baojian Li; Martin Woodle; Patrick Y Lu
Journal: Methods Mol Biol Date: 2008

2. RNA interference-mediated silencing of the respiratory syncytial virus nucleocapsid defines a potent antiviral strategy.

Authors: Rene Alvarez; Sayda Elbashir; Todd Borland; Ivanka Toudjarska; Philipp Hadwiger; Mathias John; Ingo Roehl; Svetlana Shulga Morskaya; Rick Martinello; Jeffrey Kahn; Mark Van Ranst; Ralph A Tripp; John P DeVincenzo; Rajendra Pandey; Martin Maier; Lubomir Nechev; Muthiah Manoharan; Victor Kotelianski; Rachel Meyers
Journal: Antimicrob Agents Chemother Date: 2009-06-08 Impact factor: 5.191

Review 3. Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection.

Authors: Vincent C C Cheng; Susanna K P Lau; Patrick C Y Woo; Kwok Yung Yuen
Journal: Clin Microbiol Rev Date: 2007-10 Impact factor: 26.132

4. Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.

Authors: Zhijun Wang; Zhenghong Yuan; Mitsuharu Matsumoto; Ulrich R Hengge; Yung-Fu Chang
Journal: Biochem Biophys Res Commun Date: 2005-02-04 Impact factor: 3.575

Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA.

1. Application of siRNA against SARS in the rhesus macaque model.

2. RNA interference-mediated silencing of the respiratory syncytial virus nucleocapsid defines a potent antiviral strategy.

Review 3. Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection.

4. Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice.

Review 5. Development of antiviral therapy for severe acute respiratory syndrome.

Review 6. Clinical picture, diagnosis, treatment and outcome of severe acute respiratory syndrome (SARS) in children.

Review 7. Antiviral applications of RNAi for coronavirus.

Review 8. RNA interference: from gene silencing to gene-specific therapeutics.

Review 9. Treatment and vaccines for severe acute respiratory syndrome.

Review 10. From genome to antivirals: SARS as a test tube.