Zi-qiang Luo1, Dan-dan Feng, Shao-jie Yue. 1. Department of Physiology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Abstract
OBJECTIVE: To explore the effect of shenfu injection on pulmonary surfactant synthesis. METHODS: Lung explants of adult rats were cultured in free serum DMEM. [3H] choline incorporated into lung tissue was determined by liquid scintillation system. The contents of total phospholipid (TPL) and phosphatidylcholine (PC) in lung tissue were measured by inorganic phosphorus quantitative analysis and thin-layer chromatography, respectively. RESULTS: Shenfu injection enhanced [3H] choline incorporation in a dose-dependent manner and increased the contents of TPL and PC as well as the ratio of PC/TPL. Both protein kinase C (PKC) antagonist H7 and calmodulin antagonist W7 inhibited the effects of Shenfu injection. The ginsenosides, ginsenoside Rb1, and ginsenoside Rg1 promoted the [3H] choline incorporation. CONCLUSION: Shenfu injection can enhance the synthesis of pulmonary surfactant through pathways mediated by PKC and calmodulin. The ginsenoside is an effective component in Shenfu injection.
OBJECTIVE: To explore the effect of shenfu injection on pulmonary surfactant synthesis. METHODS: Lung explants of adult rats were cultured in free serum DMEM. [3H] choline incorporated into lung tissue was determined by liquid scintillation system. The contents of total phospholipid (TPL) and phosphatidylcholine (PC) in lung tissue were measured by inorganic phosphorus quantitative analysis and thin-layer chromatography, respectively. RESULTS: Shenfu injection enhanced [3H] choline incorporation in a dose-dependent manner and increased the contents of TPL and PC as well as the ratio of PC/TPL. Both protein kinase C (PKC) antagonist H7 and calmodulin antagonist W7 inhibited the effects of Shenfu injection. The ginsenosides, ginsenosideRb1, and ginsenoside Rg1 promoted the [3H] choline incorporation. CONCLUSION: Shenfu injection can enhance the synthesis of pulmonary surfactant through pathways mediated by PKC and calmodulin. The ginsenoside is an effective component in Shenfu injection.