| Literature DB >> 12932762 |
Cáris Maroni Nunes1, Luiz Gustavo Ferraz Lima, Camila Santos Manoel, Rodrigo Norberto Pereira, Mauro Massaharu Nakano, José Fernando Garcia.
Abstract
The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples.Entities:
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Year: 2003 PMID： 12932762 DOI： 10.1016/s0014-4894(03)00111-5
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011