Literature DB >> 1292503

A new ultrasensitive bioluminogenic enzyme substrate for beta-galactosidase.

R Geiger1, E Schneider, K Wallenfels, W Miska.   

Abstract

A derivative of D-luciferin, D-luciferin-O-beta-galactoside, was synthesized and used as highly sensitive substrate for beta-galactosidase. The substrate was physicochemically characterized. Enzymatic cleavage of the new compound by beta-galactosidase was demonstrated and kinetic constants Km, Vmax, kcat and kcat/Km have been determined. The compound has been proved to be a highly sensitive substrate for beta-galactosidase, permitting a limit of detection of 3.7 x 10(-19) mol of enzyme per assay.

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Year:  1992        PMID： 1292503     DOI： 10.1515/bchm3.1992.373.2.1187

Source DB:  PubMed          Journal:  Biol Chem Hoppe Seyler        ISSN： 0177-3593


  11 in total

1.  An ImmunoChip prototype for simultaneous detection of antiepileptic drugs using an enhanced one-step homogeneous immunoassay.

Authors:  Xiaoyun Yang; Jarmila Janatova; Joetta M Juenke; Gwendolyn A McMillin; Joseph D Andrade
Journal:  Anal Biochem       Date:  2007-03-24       Impact factor: 3.365

2.  Visualizing cellular interactions with a generalized proximity reporter.

Authors:  Mark A Sellmyer; Laura Bronsart; Hiroshi Imoto; Christopher H Contag; Thomas J Wandless; Jennifer A Prescher
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-06       Impact factor: 11.205

Review 3.  Caged luciferins for bioluminescent activity-based sensing.

Authors:  Timothy A Su; Kevin J Bruemmer; Christopher J Chang
Journal:  Curr Opin Biotechnol       Date:  2019-06-11       Impact factor: 9.740

4.  In vivo imaging of hydrogen peroxide production in a murine tumor model with a chemoselective bioluminescent reporter.

Authors:  Genevieve C Van de Bittner; Elena A Dubikovskaya; Carolyn R Bertozzi; Christopher J Chang
Journal:  Proc Natl Acad Sci U S A       Date:  2010-11-29       Impact factor: 11.205

5.  Strategy for dual-analyte luciferin imaging: in vivo bioluminescence detection of hydrogen peroxide and caspase activity in a murine model of acute inflammation.

Authors:  Genevieve C Van de Bittner; Carolyn R Bertozzi; Christopher J Chang
Journal:  J Am Chem Soc       Date:  2013-01-25       Impact factor: 15.419

6.  Synthesis of novel N-acetylneuraminic acid derivatives as substrates for rapid detection of influenza virus neuraminidase.

Authors:  Wei Yang; Xiaoyu Liu; Xiaoxia Peng; Pei Li; Tianxin Wang; Guihua Tai; X James Li; Yifa Zhou
Journal:  Carbohydr Res       Date:  2012-06-21       Impact factor: 2.104

7.  Imaging beta-galactosidase activity in vivo using sequential reporter-enzyme luminescence.

Authors:  Georges von Degenfeld; Tom S Wehrman; Helen M Blau
Journal:  Methods Mol Biol       Date:  2009

Review 8.  Beyond D-luciferin: expanding the scope of bioluminescence imaging in vivo.

Authors:  Spencer T Adams; Stephen C Miller
Journal:  Curr Opin Chem Biol       Date:  2014-08-01       Impact factor: 8.822

9.  Identification of Small Molecule Inhibitors of a Mir155 Transcriptional Reporter in Th17 Cells.

Authors:  Anju Singh; Myagmarjav Dashynam; Bryan Chim; Thelma M Escobar; Xiuhuai Liu; Xin Hu; Samarjit Patnaik; Xin Xu; Noel Southall; Juan Marugan; Ajit Jadhav; Vanja Lazarevic; Stefan A Muljo; Marc Ferrer
Journal:  Sci Rep       Date:  2021-06-01       Impact factor: 4.996

10.  cybLuc: An Effective Aminoluciferin Derivative for Deep Bioluminescence Imaging.

Authors:  Wenxiao Wu; Jing Su; Chunchao Tang; Haixiu Bai; Zhao Ma; Tianchao Zhang; Zenglin Yuan; Zhenzhen Li; Wenjuan Zhou; Huateng Zhang; Zhenzhen Liu; Yue Wang; Yubin Zhou; Lupei Du; Lichuan Gu; Minyong Li
Journal:  Anal Chem       Date:  2017-04-13       Impact factor: 6.986
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