Lingyun Zheng1, Zuyu Zhou, Dachang Tao, Tingjian Lan. 1. Department of Physiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Abstract
OBJECTIVE: To observe the protective effect of berberine on cardiac myocyte injured by ischemia-reperfusion(I/R) in neonatal rats. METHODS: Cardiac myocytes from neonatal SD rat were cultured and pretreated with berberine in three different concentrations, 1.5 x 10(-6) mol/L, 1.5 x 10(-5) mol/L and 1.5 x 10(-4) mol/L, before exposure to hypoxia (95% N2-5% CO2) for 24 h and reoxygenation (95% air-5% CO2) for 1 h to create cell model of ischemia-reperfusion. Then lactate dehydrogenase (LDH) release, methylenedioxyamphetamine (MDA) release superoxide dismutase (SOD) activity were measured, and cell apoptosis was detected. RESULTS: Compared with the measurements taken in normal group, the LDH and MDA in the supernatant of the cells in ischemia and reperfusion group increased highly (P < 0.01), SOD activity decreased sharply (P < 0.01), and apoptosis of cells in ischemia groups and reperfusion groups increased highly (P < 0.01). Pretreatment of myocytes with berberine resulted in reduction in LDH and MDA release (P < 0.01) in I/R groups, attenuation of apoptosis in ischemia and reperfusion groups (P < 0.01). When the concentration of berberine was increased, these effects were getting obvious. Especially when the myocytes were pretreated with berberine at 1.5 x 10(-5) mmol/L, the cell apoptosis rates were 14.4% and 20% in ischemia group and reperfusion group, which were lower than the rates of 17.4% and 41% before pretreatment, respectively. CONCLUSION: Berberine alleviates I/R injury and attenuates apoptosis in myocytes exposed to I/R. These effects partly depend on the concentration of berberine.
OBJECTIVE: To observe the protective effect of berberine on cardiac myocyte injured by ischemia-reperfusion(I/R) in neonatal rats. METHODS: Cardiac myocytes from neonatal SD rat were cultured and pretreated with berberine in three different concentrations, 1.5 x 10(-6) mol/L, 1.5 x 10(-5) mol/L and 1.5 x 10(-4) mol/L, before exposure to hypoxia (95% N2-5% CO2) for 24 h and reoxygenation (95% air-5% CO2) for 1 h to create cell model of ischemia-reperfusion. Then lactate dehydrogenase (LDH) release, methylenedioxyamphetamine (MDA) release superoxide dismutase (SOD) activity were measured, and cell apoptosis was detected. RESULTS: Compared with the measurements taken in normal group, the LDH and MDA in the supernatant of the cells in ischemia and reperfusion group increased highly (P < 0.01), SOD activity decreased sharply (P < 0.01), and apoptosis of cells in ischemia groups and reperfusion groups increased highly (P < 0.01). Pretreatment of myocytes with berberine resulted in reduction in LDH and MDA release (P < 0.01) in I/R groups, attenuation of apoptosis in ischemia and reperfusion groups (P < 0.01). When the concentration of berberine was increased, these effects were getting obvious. Especially when the myocytes were pretreated with berberine at 1.5 x 10(-5) mmol/L, the cell apoptosis rates were 14.4% and 20% in ischemia group and reperfusion group, which were lower than the rates of 17.4% and 41% before pretreatment, respectively. CONCLUSION:Berberine alleviates I/R injury and attenuates apoptosis in myocytes exposed to I/R. These effects partly depend on the concentration of berberine.