OBJECTIVE: To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. METHODS: Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture. RESULTS: CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. CONCLUSION: CKLF1 can promote the proliferation and differentiation of bone marrow cells.
OBJECTIVE: To investigate the activity of CKLF1 on the proliferation and differentiation of bone marrow cells. METHODS:Human low density bone marrow cells and mouse bone marrow cells were plated in 96-well microplate and supernatants from transfected COS-7 cell culture were added. The cell proliferation was assayed by MTT method after 5 days incubation. The enhancing effect of CKLF1 on the colony formation of human hematopoietic progenitor cells was identified in semi-solid culture. RESULTS:CKLF1 has obvious enhancing effect on both human and mouse bone marrow cells, it can stimulate the colony formation of human hematopoietic stem cells and has synergistic action with GM-CSF. CONCLUSION:CKLF1 can promote the proliferation and differentiation of bone marrow cells.