Literature DB >> 12904425

Clinical impact of a PCR assay for identification of Staphylococcus aureus and determination of methicillin resistance directly from blood cultures.

M Hallin1, N Maes, B Byl, F Jacobs, Y De Gheldre, M J Struelens.   

Abstract

We evaluated the clinical usefulness of a PCR assay that discriminates Staphylococcus aureus from coagulase-negative staphylococci and detects methicillin resistance on blood cultures by measuring the adaptation of antimicrobial therapy based on the PCR results. Only 7 of 28 patients (25%) benefited from a modification of antibiotic therapy based on the PCR results, since empirical therapy was appropriate in a majority of cases.

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Year:  2003        PMID： 12904425      PMCID： PMC179783          DOI： 10.1128/JCM.41.8.3942-3944.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

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Journal:  Clin Infect Dis       Date:  2001-05-15       Impact factor: 9.079

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Journal:  J Clin Microbiol       Date:  2001-12       Impact factor: 5.948

5.  Mortality associated with nosocomial bacteremia due to methicillin-resistant Staphylococcus aureus.

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Review 9.  Nosocomial bloodstream infections: organisms, risk factors, and implications.

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  11 in total

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Journal:  Asian Pac J Trop Biomed       Date:  2014-05

3.  Community-acquired methicillin-resistant Staphylococcus aureus clones circulating in Belgium from 2005 to 2009: changing epidemiology.

Authors:  J Brauner; M Hallin; A Deplano; R De Mendonça; C Nonhoff; R De Ryck; S Roisin; M J Struelens; O Denis
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4.  Validation of pulsed-field gel electrophoresis and spa typing for long-term, nationwide epidemiological surveillance studies of Staphylococcus aureus infections.

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5.  Hemoculture and Direct Sputum Detection of mecA-Mediated Methicillin-Resistant Staphylococcus aureus by Loop-Mediated Isothermal Amplification in Combination With a Lateral-Flow Dipstick.

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6.  Accuracy and potential usefulness of triplex real-time PCR for improving antibiotic treatment of patients with blood cultures showing clustered gram-positive cocci on direct smears.

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7.  Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens.

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Review 9.  Effectiveness of Practices To Increase Timeliness of Providing Targeted Therapy for Inpatients with Bloodstream Infections: a Laboratory Medicine Best Practices Systematic Review and Meta-analysis.

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10.  Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor for Detection of Staphylococcus aureus and Identification of Methicillin-Resistant S. aureus.

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