Literature DB >> 12904406

Quantitative real-time PCR assay for detection of Ehrlichia chaffeensis.

Amanda D Loftis1, Robert F Massung, Michael L Levin.   

Abstract

A real-time PCR assay was developed for the detection of Ehrlichia chaffeensis. The assay is species specific and provides quantitative results in the range 10 to 10(10) gene copies. The assay is not inhibited by the presence of tick, human, or mouse DNA and is compatible with high sample throughput. The assay was compared with previously described assays for E. chaffeensis.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12904406      PMCID： PMC179802          DOI： 10.1128/JCM.41.8.3870-3872.2003

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

1.  Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila.

Authors:  J S Dumler; A F Barbet; C P Bekker; G A Dasch; G H Palmer; S C Ray; Y Rikihisa; F R Rurangirwa
Journal:  Int J Syst Evol Microbiol       Date:  2001-11       Impact factor: 2.747

2.  Experimental transmission of Ehrlichia chaffeensis (Rickettsiales: Ehrlichieae) among white-tailed deer by Amblyomma americanum (Acari: Ixodidae).

Authors:  S A Ewing; J E Dawson; A A Kocan; R W Barker; C K Warner; R J Panciera; J C Fox; K M Kocan; E F Blouin
Journal:  J Med Entomol       Date:  1995-05       Impact factor: 2.278

Review 3.  The human ehrlichioses in the United States.

Authors:  J H McQuiston; C D Paddock; R C Holman; J E Childs
Journal:  Emerg Infect Dis       Date:  1999 Sep-Oct       Impact factor: 6.883

4.  Substrate utilization by Ehrlichia sennetsu and Ehrlichia risticii separated from host constituents by renografin gradient centrifugation.

Authors:  E Weiss; G A Dasch; Y H Kang; H N Westfall
Journal:  J Bacteriol       Date:  1988-11       Impact factor: 3.490

5.  Amblyomma americanum: a potential vector of human ehrlichiosis.

Authors:  B E Anderson; K G Sims; J G Olson; J E Childs; J F Piesman; C M Happ; G O Maupin; B J Johnson
Journal:  Am J Trop Med Hyg       Date:  1993-08       Impact factor: 2.345

6.  Detection of the etiologic agent of human ehrlichiosis by polymerase chain reaction.

Authors:  B E Anderson; J W Sumner; J E Dawson; T Tzianabos; C R Greene; J G Olson; D B Fishbein; M Olsen-Rasmussen; B P Holloway; E H George
Journal:  J Clin Microbiol       Date:  1992-04       Impact factor: 5.948

7.  Isolation and characterization of an Ehrlichia sp. from a patient diagnosed with human ehrlichiosis.

Authors:  J E Dawson; B E Anderson; D B Fishbein; J L Sanchez; C S Goldsmith; K H Wilson; C W Duntley
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

8.  Characterization of the rRNA genes of Ehrlichia chaffeensis and Anaplasma phagocytophila.

Authors:  Robert F Massung; Kemba Lee; Michael Mauel; Asiya Gusa
Journal:  DNA Cell Biol       Date:  2002-08       Impact factor: 3.311

9.  Ehrlichia muris sp. nov., identified on the basis of 16S rRNA base sequences and serological, morphological, and biological characteristics.

Authors:  B Wen; Y Rikihisa; J Mott; P A Fuerst; M Kawahara; C Suto
Journal:  Int J Syst Bacteriol       Date:  1995-04

10.  Serologic diagnosis of human ehrlichiosis using two Ehrlichia canis isolates.

Authors:  J E Dawson; Y Rikihisa; S A Ewing; D B Fishbein
Journal:  J Infect Dis       Date:  1991-03       Impact factor: 5.226
View more
  17 in total

Review 1.  Response of the clinical microbiology laboratory to emerging (new) and reemerging infectious diseases.

Authors:  Franklin R Cockerill; Thomas F Smith
Journal:  J Clin Microbiol       Date:  2004-06       Impact factor: 5.948

2.  Prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii in Amblyomma americanum and Dermacentor variabilis collected from southeastern Virginia, 2010-2011.

Authors:  Chelsea L Wright; Holly D Gaff; Wayne L Hynes
Journal:  Ticks Tick Borne Dis       Date:  2014-08-22       Impact factor: 3.744

Review 3.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

4.  Detection of bacterial agents in Amblyomma americanum (Acari: Ixodidae) from Georgia, USA, and the use of a multiplex assay to differentiate Ehrlichia chaffeensis and Ehrlichia ewingii.

Authors:  L F Killmaster; A D Loftis; G E Zemtsova; M L Levin
Journal:  J Med Entomol       Date:  2014-07       Impact factor: 2.278

5.  Tick-borne rickettsial pathogens in ticks and small mammals in Korea.

Authors:  Chul-Min Kim; Ying-Hua Yi; Do-Hyeon Yu; Mi-Jin Lee; Mae-Rim Cho; Atul R Desai; Smriti Shringi; Terry A Klein; Heung-Chul Kim; Jin-Won Song; Luck-Ju Baek; Sung-Tae Chong; Monica L O'guinn; John S Lee; In-Yong Lee; Jin-Ho Park; Janet Foley; Joon-Seok Chae
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

6.  Multiplex detection of Ehrlichia and Anaplasma species pathogens in peripheral blood by real-time reverse transcriptase-polymerase chain reaction.

Authors:  Kamesh R Sirigireddy; Roman R Ganta
Journal:  J Mol Diagn       Date:  2005-05       Impact factor: 5.568

7.  Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene.

Authors:  C Kuyler Doyle; Marcelo B Labruna; Edward B Breitschwerdt; Yi-Wei Tang; Richard E Corstvet; Barbara C Hegarty; Karen C Bloch; Ping Li; David H Walker; Jere W McBride
Journal:  J Mol Diagn       Date:  2005-10       Impact factor: 5.568

8.  Development of a p28-based PCR assay for Ehrlichia chaffeensis.

Authors:  Elizabeth R Wagner; William G Bremer; Yasuko Rikihisa; S A Ewing; Glen R Needham; Ahmet Unver; Xueqi Wang; Roger W Stich
Journal:  Mol Cell Probes       Date:  2004-04       Impact factor: 2.365

9.  Ehrlichia and spotted fever group Rickettsiae surveillance in Amblyomma americanum in Virginia through use of a novel six-plex real-time PCR assay.

Authors:  David N Gaines; Darwin J Operario; Suzanne Stroup; Ellen Stromdahl; Chelsea Wright; Holly Gaff; James Broyhill; Joshua Smith; Douglas E Norris; Tyler Henning; Agape Lucas; Eric Houpt
Journal:  Vector Borne Zoonotic Dis       Date:  2014-04-18       Impact factor: 2.133

10.  Experimental infection of dairy calves with Ehrlichia chaffeensis.

Authors:  Jose R C Delos Santos; Kirsten Boughan; William G Bremer; Brian Rizzo; John J Schaefer; Yasuko Rikihisa; Glen R Needham; L A Capitini; David E Anderson; Michael Oglesbee; S A Ewing; Roger W Stich
Journal:  J Med Microbiol       Date:  2007-12       Impact factor: 2.472
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.