Y Chen1, Y Zhang, R Sun, Q Song, C Di, D Ma. 1. Department of Immunology, School of Basic Medical Sciences, Peking University, Beijing 100083, China. yingyu_chen@yahoo.com
Abstract
OBJECTIVE: To obtain mouse anti-human TFAR19 monoclonal antibodies for further study of the structure and function of the apoptosis-related protein TFAR19. METHODS: BALB/c mice were immunized with recombinant human TFAR19, hybridoma cells were screened by cell fusion and subcloning approach. The monoclonal antibodies were identified by ELISA and Western blotting. RESULTS: Three hybridoma cells (C1, C10, 2C12) stable in secreting anti-TFAR19 monoclonal antibodies were obtained. The monoclonal antibodies showed high specificity and high titer to TFAR19 with various affinity. All of them belong to IgG1 subclass. CONCLUSIONS: These monoclonal antibodies could bind specifically to TFAR19 protein.
OBJECTIVE: To obtain mouse anti-humanTFAR19 monoclonal antibodies for further study of the structure and function of the apoptosis-related protein TFAR19. METHODS: BALB/c mice were immunized with recombinant humanTFAR19, hybridoma cells were screened by cell fusion and subcloning approach. The monoclonal antibodies were identified by ELISA and Western blotting. RESULTS: Three hybridoma cells (C1, C10, 2C12) stable in secreting anti-TFAR19 monoclonal antibodies were obtained. The monoclonal antibodies showed high specificity and high titer to TFAR19 with various affinity. All of them belong to IgG1 subclass. CONCLUSIONS: These monoclonal antibodies could bind specifically to TFAR19 protein.