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Literature DB >> 12903243

Construction of an efficient expression vector for coupled transcription/translation in a wheat germ cell-free system.

T Sawasaki¹, Y Hasegawa, M Tsuchimochi, Y Kasahara, Y Endo.

Abstract

Using the expression vector, pEU, which we have constructed, highly efficient in vitro protein synthesis can be achieved: The system works for 150 hours and without further template addition once the reaction has started, yielding 5 mg of enzymatically active protein in a 1 ml reaction.

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Year: 2000 PMID： 12903243 DOI： 10.1093/nass/44.1.9

Source DB: PubMed Journal: Nucleic Acids Symp Ser ISSN： 0261-3166

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Cited

10 in total

1. Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa.

Authors: Elitza S Sevova; Michael A Goren; Kevin J Schwartz; Fong-Fu Hsu; John Turk; Brian G Fox; James D Bangs
Journal: J Biol Chem Date: 2010-05-10 Impact factor: 5.157

2. Using translational enhancers to increase transgene expression in Drosophila.

Authors: Barret D Pfeiffer; James W Truman; Gerald M Rubin
Journal: Proc Natl Acad Sci U S A Date: 2012-04-09 Impact factor: 11.205

3. Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein.

Authors: Bo Li; Shin-Ichi Makino; Emily T Beebe; Daisuke Urano; David J Aceti; Tina M Misenheimer; Jonathan Peters; Brian G Fox; Alan M Jones
Journal: Protein Expr Purif Date: 2016-05-06 Impact factor: 1.650

4. Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.

Authors: David J Aceti; Craig A Bingman; Russell L Wrobel; Ronnie O Frederick; Shin-Ichi Makino; Karl W Nichols; Sarata C Sahu; Lai F Bergeman; Paul G Blommel; Claudia C Cornilescu; Katarzyna A Gromek; Kory D Seder; Soyoon Hwang; John G Primm; Grzegorz Sabat; Frank C Vojtik; Brian F Volkman; Zsolt Zolnai; George N Phillips; John L Markley; Brian G Fox
Journal: J Struct Funct Genomics Date: 2015-04-09

Construction of an efficient expression vector for coupled transcription/translation in a wheat germ cell-free system.

1. Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa.

2. Using translational enhancers to increase transgene expression in Drosophila.

3. Cell-free translation and purification of Arabidopsis thaliana regulator of G signaling 1 protein.

4. Expression platforms for producing eukaryotic proteins: a comparison of E. coli cell-based and wheat germ cell-free synthesis, affinity and solubility tags, and cloning strategies.

5. Amino acid determinants of substrate selectivity in the Trypanosoma brucei sphingolipid synthase family.

6. Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex.

Review 7. Cell-free translation of integral membrane proteins into unilamelar liposomes.

8. Protein structural biology using cell-free platform from wheat germ.

9. Identification and characterization of a set of monocot BAHD monolignol transferases.

10. Biofoundry-assisted expression and characterization of plant proteins.