| Literature DB >> 12898427 |
Hiromitsu Yoshimura, Koko Sugawara, Masako Saito, Shiuji Saito, Shigeru Murakami, Noriyuki Miyata, Akira Kawashima, Shigeo Morimoto, Nannan Gao, Xingguo Zhang, Junshan Yang.
Abstract
The mechanism of action of the aerial parts of Clerodendranthus spicatus (Thunb.) C.Y. Wu, [syn. Orthosiphon aristatus (Blume) Miq,] a medicinal plant used in China to treat human renal disease, was investigated. The aqueous and methanol crude extracts exhibited dose-dependent inhibitory activity on 125I-TGF-beta1 binding to its receptor in Balb/c 3T3 cells. Subsequent bioassay-guided fractionation led to identification of two known triterpenoidal constituents, ursolic and oleanolic acids. Ursolic and oleanolic acids inhibited the binding of 125I-TGF-beta1 to its receptor with IC 50 values of 6.9 +/- 0.8 and 21.0 +/- 2.3 microM, respectively. The results suggest that TGF-beta1 antagonistic activity is responsible, at least in part, for the therapeutic efficacy of this plant to treat humans with renal disease.Entities:
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Year: 2003 PMID： 12898427 DOI： 10.1055/s-2003-41110
Source DB: PubMed Journal: Planta Med ISSN： 0032-0943 Impact factor: 3.352