BACKGROUND AND AIMS: Human VAT-1 (hVAT-1) is a homologue of the synaptic vesicle membrane protein of Torpedo californica. Its coding gene is located near the BRCA1 locus and thus hVAT-1 may be linked to an inherited predisposition to breast and ovary cancer. However, the hVAT-1 protein expression pattern in normal epithelial tissues such as skin, mammary gland and ovary, as well as in tumours of the mammary gland and ovary, has not been studied. METHODS: To address this issue, an immunohistological analysis of biopsies of normal epidermis and lesional epidermis of bullous pemphigoid and pemphigus vulgaris patients was undertaken. RESULTS: hVAT-1-expression was observed in basal keratinocytes of lesional epidermis of bullous pemphigoid patients but not in normal epidermis or in lesional epidermis of pemphigus vulgaris patients. Moreover, hVAT-1 expression in HaCaT cells was found to be calcium-dependent. Normal and malignant mammary and ovary epithelium were found to be hVAT-1-negative. CONCLUSIONS: Our results indicate that hVAT-1 exerts a specific function in keratinocyte physiology, in particular in calcium-regulated processes, with no evident deregulation in malignancies of the breast and ovary.
BACKGROUND AND AIMS: HumanVAT-1 (hVAT-1) is a homologue of the synaptic vesicle membrane protein of Torpedo californica. Its coding gene is located near the BRCA1 locus and thus hVAT-1 may be linked to an inherited predisposition to breast and ovary cancer. However, the hVAT-1 protein expression pattern in normal epithelial tissues such as skin, mammary gland and ovary, as well as in tumours of the mammary gland and ovary, has not been studied. METHODS: To address this issue, an immunohistological analysis of biopsies of normal epidermis and lesional epidermis of bullous pemphigoid and pemphigus vulgarispatients was undertaken. RESULTS:hVAT-1-expression was observed in basal keratinocytes of lesional epidermis of bullous pemphigoid patients but not in normal epidermis or in lesional epidermis of pemphigus vulgarispatients. Moreover, hVAT-1 expression in HaCaT cells was found to be calcium-dependent. Normal and malignant mammary and ovary epithelium were found to be hVAT-1-negative. CONCLUSIONS: Our results indicate that hVAT-1 exerts a specific function in keratinocyte physiology, in particular in calcium-regulated processes, with no evident deregulation in malignancies of the breast and ovary.
Authors: Bai-Yu Lee; Deepa Jethwaney; Birgit Schilling; Daniel L Clemens; Bradford W Gibson; Marcus A Horwitz Journal: Mol Cell Proteomics Date: 2009-10-07 Impact factor: 5.911
Authors: Kenzie D M Birse; Amy L Cole; Taha Hirbod; Lyle McKinnon; Terry B Ball; Garrett R Westmacott; Joshua Kimani; Frank Plummer; Alexander M Cole; Adam Burgener; Kristina Broliden Journal: PLoS One Date: 2015-06-19 Impact factor: 3.240
Authors: Carolin M-L Gleissner; Carolin L Pyka; Wolfgang Heydenreuter; Thomas F Gronauer; Carina Atzberger; Vadim S Korotkov; Weiting Cheng; Stephan M Hacker; Angelika M Vollmar; Simone Braig; Stephan A Sieber Journal: ACS Cent Sci Date: 2019-06-18 Impact factor: 14.553