Literature DB >> 1289805

Effects of base damages on DNA replication--mechanism of preferential purine nucleotide insertion opposite abasic site in template DNA.

H Ide1, H Murayama, A Murakami, T Morii, K Makino.   

Abstract

DNA polymerase preferentially inserts purine nucleotides opposite non-instructive lesions such as abasic sites during DNA replication. In order to elucidate the mechanism of the preferential insertion, a DNA template containing a model abasic site and primers containing 4 different nucleotides (A,G,C,T) at primer terminus were synthesized. The stability of the primer terminus nucleotide placed opposite the abasic site was evaluated on the basis of its sensitivity to 3'-5' exonuclease associated with DNA polymerase.

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Year:  1992        PMID： 1289805

Source DB:  PubMed          Journal:  Nucleic Acids Symp Ser        ISSN： 0261-3166


  4 in total

Review 1.  The molecular etiology and prevention of estrogen-initiated cancers: Ockham's Razor: Pluralitas non est ponenda sine necessitate. Plurality should not be posited without necessity.

Authors:  Ercole Cavalieri; Eleanor Rogan
Journal:  Mol Aspects Med       Date:  2013-08-30

2.  Replication bypass and mutagenic effect of alpha-deoxyadenosine site-specifically incorporated into single-stranded vectors.

Authors:  H Shimizu; R Yagi; Y Kimura; K Makino; H Terato; Y Ohyama; H Ide
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

3.  On the mechanism of preferential incorporation of dAMP at abasic sites in translesional DNA synthesis. Role of proofreading activity of DNA polymerase and thermodynamic characterization of model template-primers containing an abasic site.

Authors:  H Ide; H Murayama; S Sakamoto; K Makino; K Honda; H Nakamuta; M Sasaki; N Sugimoto
Journal:  Nucleic Acids Res       Date:  1995-01-11       Impact factor: 16.971

4.  Serial analysis of mutation spectra (SAMS): a new approach for the determination of mutation spectra of site-specific DNA damage and their sequence dependence.

Authors:  Huafeng Fang; John-Stephen Taylor
Journal:  Nucleic Acids Res       Date:  2008-09-23       Impact factor: 16.971
  4 in total

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