Literature DB >> 12897026

Conversion of Lactobacillus pentosus D-lactate dehydrogenase to a D-hydroxyisocaproate dehydrogenase through a single amino acid replacement.

Chizuka Tokuda1, Yoshiro Ishikura, Mayu Shigematsu, Hiroyuki Mutoh, Shino Tsuzuki, Yusaku Nakahira, Yusuke Tamura, Takeshi Shinoda, Kazuhito Arai, O Takahashi, Hayao Taguchi.   

Abstract

The single amino acid replacement of Tyr52 with Leu drastically increased the activity of Lactobacillus pentosus NAD-dependent D-lactate dehydrogenase toward larger aliphatic or aromatic 2-ketoacid substrates by 3 or 4 orders of magnitude and decreased the activity toward pyruvate by about 30-fold, converting the enzyme into a highly active D-2-hydroxyisocaproate dehydrogenase.

Entities:  

Mesh：

Substances：

Year:  2003        PMID： 12897026      PMCID： PMC166478          DOI： 10.1128/JB.185.16.5023-5026.2003

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  33 in total

1.  Glutamate 264 modulates the pH dependence of the NAD(+)-dependent D-lactate dehydrogenase.

Authors:  S Kochhar; N Chuard; H Hottinger
Journal:  J Biol Chem       Date:  1992-10-05       Impact factor: 5.157

2.  Structural relationship between the vancomycin resistance protein VanH and 2-hydroxycarboxylic acid dehydrogenases.

Authors:  M Arthur; C Molinas; S Dutka-Malen; P Courvalin
Journal:  Gene       Date:  1991-07-15       Impact factor: 3.688

Review 3.  NAD(+)-dependent formate dehydrogenase.

Authors:  V O Popov; V S Lamzin
Journal:  Biochem J       Date:  1994-08-01       Impact factor: 3.857

4.  Primary structure, physicochemical properties, and chemical modification of NAD(+)-dependent D-lactate dehydrogenase. Evidence for the presence of Arg-235, His-303, Tyr-101, and Trp-19 at or near the active site.

Authors:  S Kochhar; P E Hunziker; P Leong-Morgenthaler; H Hottinger
Journal:  J Biol Chem       Date:  1992-04-25       Impact factor: 5.157

5.  Improved specificity toward substrates with positively charged side chains by site-directed mutagenesis of the L-lactate dehydrogenase of Bacillus stearothermophilus.

Authors:  J K Hogan; C A Pittol; J B Jones; M Gold
Journal:  Biochemistry       Date:  1995-04-04       Impact factor: 3.162

6.  D-lactate dehydrogenase is a member of the D-isomer-specific 2-hydroxyacid dehydrogenase family. Cloning, sequencing, and expression in Escherichia coli of the D-lactate dehydrogenase gene of Lactobacillus plantarum.

Authors:  H Taguchi; T Ohta
Journal:  J Biol Chem       Date:  1991-07-05       Impact factor: 5.157

7.  Molecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by vancomycin resistance proteins VanH and VanA.

Authors:  T D Bugg; G D Wright; S Dutka-Malen; M Arthur; P Courvalin; C T Walsh
Journal:  Biochemistry       Date:  1991-10-29       Impact factor: 3.162

8.  Histidine 296 is essential for the catalysis in Lactobacillus plantarum D-lactate dehydrogenase.

Authors:  H Taguchi; T Ohta
Journal:  J Biol Chem       Date:  1993-08-25       Impact factor: 5.157

9.  NAD(+)-dependent D-2-hydroxyisocaproate dehydrogenase of Lactobacillus delbrueckii subsp. bulgaricus. Gene cloning and enzyme characterization.

Authors:  N Bernard; K Johnsen; T Ferain; D Garmyn; P Hols; J J Holbrook; J Delcour
Journal:  Eur J Biochem       Date:  1994-09-01

10.  Crystal structure of a NAD-dependent D-glycerate dehydrogenase at 2.4 A resolution.

Authors:  J D Goldberg; T Yoshida; P Brick
Journal:  J Mol Biol       Date:  1994-03-04       Impact factor: 5.469
View more
  9 in total

1.  Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile.

Authors:  Jihoe Kim; Daniel Darley; Thorsten Selmer; Wolfgang Buckel
Journal:  Appl Environ Microbiol       Date:  2006-09       Impact factor: 4.792

2.  The D-2-hydroxyacid dehydrogenase incorrectly annotated PanE is the sole reduction system for branched-chain 2-keto acids in Lactococcus lactis.

Authors:  Emilie Chambellon; Liesbeth Rijnen; Frédérique Lorquet; Christophe Gitton; Johan E T van Hylckama Vlieg; Jeroen A Wouters; Mireille Yvon
Journal:  J Bacteriol       Date:  2008-12-01       Impact factor: 3.490

3.  Construction of a chimeric biosynthetic pathway for the de novo biosynthesis of rosmarinic acid in Escherichia coli.

Authors:  Sarah E Bloch; Claudia Schmidt-Dannert
Journal:  Chembiochem       Date:  2014-09-09       Impact factor: 3.164

4.  Efficient conversion of phenylpyruvic acid to phenyllactic acid by using whole cells of Bacillus coagulans SDM.

Authors:  Zhaojuan Zheng; Cuiqing Ma; Chao Gao; Fengsong Li; Jiayang Qin; Haiwei Zhang; Kai Wang; Ping Xu
Journal:  PLoS One       Date:  2011-04-20       Impact factor: 3.240

5.  Diverse allosteric and catalytic functions of tetrameric d-lactate dehydrogenases from three Gram-negative bacteria.

Authors:  Nayuta Furukawa; Akimasa Miyanaga; Misato Togawa; Masahiro Nakajima; Hayao Taguchi
Journal:  AMB Express       Date:  2014-10-28       Impact factor: 3.298

Review 6.  In vitro Engineering of Novel Bioactivity in the Natural Enzymes.

Authors:  Vishvanath Tiwari
Journal:  Front Chem       Date:  2016-10-07       Impact factor: 5.221

7.  Enzymatic characterization of D-lactate dehydrogenase and application in alanine aminotransferase activity assay kit.

Authors:  Yi Sun; Guosheng Gao; Ting Cai
Journal:  Bioengineered       Date:  2021-12       Impact factor: 3.269

8.  Highly stereoselective biosynthesis of (R)-α-hydroxy carboxylic acids through rationally re-designed mutation of D-lactate dehydrogenase.

Authors:  Zhaojuan Zheng; Binbin Sheng; Chao Gao; Haiwei Zhang; Tong Qin; Cuiqing Ma; Ping Xu
Journal:  Sci Rep       Date:  2013-12-02       Impact factor: 4.379

9.  Substrate Specificity and Allosteric Regulation of a D-Lactate Dehydrogenase from a Unicellular Cyanobacterium are Altered by an Amino Acid Substitution.

Authors:  Shoki Ito; Masahiro Takeya; Takashi Osanai
Journal:  Sci Rep       Date:  2017-11-08       Impact factor: 4.379
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.