Literature DB >> 12892894

Purification and characterization of a novel lignin peroxidase from white-rot fungus Phanerochaete sordida YK-624.

Mutsumi Sugiura1, Hirofumi Hirai, Tomoaki Nishida.   

Abstract

We characterized kinetics and substrate oxidation of a novel lignin peroxidase (YK-LiP) isolated from white-rot fungus Phanerochaete sordida YK-624. YK-LiP enzyme was identified and purified to homogeneity by anion-exchange chromatography and gel permeation chromatography. The molecular mass of YK-LiP was approximately 50 kDa, and the absorption spectrum of YK-LiP was almost the same as that of the LiP (Pc-LiP) from Phanerochaete chrysosporium. Steady-state kinetics of veratryl alcohol oxidation by YK-LiP (unlike that by Pc-LiP) revealed a bi-reactant sequential mechanism, although reactivity of YK-LiP to various monomeric substituted aromatic compounds was similar to that of Pc-LiP. Degradation of dimeric lignin model compounds was more effective by YK-LiP than by Pc-LiP, and the oxidation rate of sinapyl alcohol oligomer by YK-LiP was much faster than that by Pc-LiP.

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Year:  2003        PMID： 12892894     DOI： 10.1016/S0378-1097(03)00447-6

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  6 in total

1.  Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor.

Authors:  Md Zahangir Alam; Mariatul F Mansor; K C A Jalal
Journal:  J Ind Microbiol Biotechnol       Date:  2009-03-04       Impact factor: 3.346

2.  Effective removal of endocrine-disrupting compounds by lignin peroxidase from the white-rot fungus Phanerochaete sordida YK-624.

Authors:  Jianqiao Wang; Nayumi Majima; Hirofumi Hirai; Hirokazu Kawagishi
Journal:  Curr Microbiol       Date:  2011-12-28       Impact factor: 2.188

Review 3.  Biomass waste-to-energy valorisation technologies: a review case for banana processing in Uganda.

Authors:  Robert Gumisiriza; Joseph Funa Hawumba; Mackay Okure; Oliver Hensel
Journal:  Biotechnol Biofuels       Date:  2017-01-03       Impact factor: 6.040

4.  In silico exploration of lignin peroxidase for unraveling the degradation mechanism employing lignin model compounds.

Authors:  Anil Kumar Singh; Sudheer Kumar Katari; Amineni Umamaheswari; Abhay Raj
Journal:  RSC Adv       Date:  2021-04-20       Impact factor: 3.361

5.  Characterization of purified and xerogel immobilized novel lignin peroxidase produced from Trametes versicolor IBL-04 using solid state medium of corncobs.

Authors:  Muhammad Asgher; Hafiz Muhammad Nasir Iqbal; Muhammad Irshad
Journal:  BMC Biotechnol       Date:  2012-08-03       Impact factor: 2.563

6.  Production of Oxidative and Hydrolytic Enzymes by Coprinus cinereus (Schaeff.) Gray from Sisal Wastes Supplemented with Cow Dung Manure.

Authors:  Prosper Raymond; Anthony Manoni Mshandete; Amelia Kajumulo Kivaisi
Journal:  Biotechnol Res Int       Date:  2015-11-19
  6 in total

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