Literature DB >> 12889813

Purification and properties of a heat-stable inulin fructotransferase from Arthrobacter ureafaciens.

Kazutomo Haraguchi1, Mitsuru Yoshida, Ken'ichi Ohtsubo.   

Abstract

An inulin fructotransferase producing difructose dianhydride I (EC 2.4.1.200) was purified from Arthrobacter ureafaciens A51-1. It had maximum activity at pH 5.5 and 45 degrees C, and was stable up to 80 degrees C. This is the highest thermal stability for this enzyme reported to date. The molecular mass was estimated to be 38000 by SDS-PAGE, and 61000 by gel filtration. It was therefore estimated to be a dimer.

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Year:  2003        PMID: 12889813     DOI: 10.1023/a:1024138428965

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN: 0141-5492            Impact factor:   2.461


  2 in total

1.  Two Types of Inulin Fructotransferases.

Authors:  Kazutomo Haraguchi
Journal:  Materials (Basel)       Date:  2011-09-07       Impact factor: 3.623

Review 2.  Chemical and enzymatic approaches to carbohydrate-derived spiroketals: di-D-fructose dianhydrides (DFAs).

Authors:  M Isabel García-Moreno; Juan M Benito; Carmen Ortiz Mellet; José M García Fernández
Journal:  Molecules       Date:  2008-08-12       Impact factor: 4.411

  2 in total

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