Liquid chromatographic separation of the enantiomers of beta-amino acids on a ligand exchange chiral stationary phase.

A liquid chromatographic ligand exchange chiral stationary phase (CSP) derived from (S)-leucinol was applied in the separation of the enantiomers of 12 beta-amino acids. The resolution was quite successful especially for the enantiomers of beta-amino acids containing aromatic functional group in the side chain. The chromatographic resolution behaviors were dependent on the organic modifier and Cu(II) concentration in aqueous mobile phase and the column temperature. Copyright 2003 John Wiley & Sons, Ltd.