Literature DB >> 1288416

Synergism between Clostridium thermocellum cellulases cloned in Escherichia coli.

K Tuka1, V V Zverlov, G A Velikodvorskaya.   

Abstract

We have obtained a synergistic effect during degradation of Avicel and filter paper by Clostridium thermocellum cellulases (two endoglucanases and one cellobiohydrolase) cloned in Escherichia coli. The highest degree of synergism was found at early stages of reaction, during the first 20 h: 2.5 and 2.9 on Avicel and filter paper, respectively. During combined action of all three cellulases the main product is cellobiose.

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Year:  1992        PMID： 1288416     DOI： 10.1007/bf02921671

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  9 in total

1.  Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum.

Authors:  E A Johnson; M Sakajoh; G Halliwell; A Madia; A L Demain
Journal:  Appl Environ Microbiol       Date:  1982-05       Impact factor: 4.792

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Cloning and expression of Clostridium thermocellum genes coding for thermostable exoglucanases (cellobiohydrolases) in Escherichia coli cells.

Authors:  K Tuka; V V Zverlov; B K Bumazkin; G A Velikodvorskaya
Journal:  Biochem Biophys Res Commun       Date:  1990-06-29       Impact factor: 3.575

4.  The role of cellulase concentration in determining the degree of synergism in the hydrolysis of microcrystalline cellulose.

Authors:  J Woodward; M Lima; N E Lee
Journal:  Biochem J       Date:  1988-11-01       Impact factor: 3.857

5.  [Cloning and expression of the structural gene of cellulolytic complex endoglucanase of Clostridium thermocellum phi 7 in Escherichia coli cells].

Authors:  E S Piruzian; M A Mogutov; G A Velikodvorskaia; V K Akimenko
Journal:  Dokl Akad Nauk SSSR       Date:  1985

6.  Substrate specificities of exo- and endo-type cellulases in the hydrolysis of beta-(1----3)- and beta-(1----4)-mixed D-glucans.

Authors:  T Kanda; H Yatomi; S Makishima; Y Amano; K Nisizawa
Journal:  J Biochem       Date:  1989-01       Impact factor: 3.387

7.  Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli.

Authors:  B K Bumazkin; G A Velikodvorskaya; K Tuka; M A Mogutov
Journal:  Biochem Biophys Res Commun       Date:  1990-03-30       Impact factor: 3.575

8.  Organization and distribution of the cellulosome in Clostridium thermocellum.

Authors:  E A Bayer; E Setter; R Lamed
Journal:  J Bacteriol       Date:  1985-08       Impact factor: 3.490

9.  The cellulase of Penicillium pinophilum. Synergism between enzyme components in solubilizing cellulose with special reference to the involvement of two immunologically distinct cellobiohydrolases.

Authors:  T M Wood; S I McCrae
Journal:  Biochem J       Date:  1986-02-15       Impact factor: 3.857
  9 in total
  2 in total

Review 1.  Cellulase, clostridia, and ethanol.

Authors:  Arnold L Demain; Michael Newcomb; J H David Wu
Journal:  Microbiol Mol Biol Rev       Date:  2005-03       Impact factor: 11.056

2.  Genomic and secretomic analyses reveal unique features of the lignocellulolytic enzyme system of Penicillium decumbens.

Authors:  Guodong Liu; Lei Zhang; Xiaomin Wei; Gen Zou; Yuqi Qin; Liang Ma; Jie Li; Huajun Zheng; Shengyue Wang; Chengshu Wang; Luying Xun; Guo-Ping Zhao; Zhihua Zhou; Yinbo Qu
Journal:  PLoS One       Date:  2013-02-01       Impact factor: 3.240
  2 in total

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