Literature DB >> 12884075

Molecular and serological characterization of group a rotavirus isolates obtained from hospitalized children in Goiânia, Brazil, 1998-2000.

M B L D Souza1, M L Rácz, J P G Leite, C M A Soares, R M B Martins, V Munford, D D P Cardoso.   

Abstract

Fecal samples positive for rotavirus group A ( n=120) were analyzed by enzyme immunoassay-monoclonal antibody (EIA-MAb) serotyping and/or reverse transcription/multiplex polymerase chain reaction (PCR) amplification to determine the prevalence of the [P] and G genotypes. The most prevalent G genotype/serotype detected was G1 (76.7%), followed by G2 (5.0%). Six samples were characterized as G9 by multiplex PCR, and one sample was characterized as G3 by EIA-MAb. The combinations of [P] and G genotypes found were P[8] and G1 (20.8%), P[6] and G1 (10.8%), P[6] and G9 (4.2%), P[8] and G2 (1.7%), and P[6] and G2 (0.8%). The diversity of rotavirus group A [P] and G genotypes/serotypes reinforces the need for continuous characterization of rotaviruses circulating in populations in Brazil.

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Year:  2003        PMID: 12884075     DOI: 10.1007/s10096-003-0952-0

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN: 0934-9723            Impact factor:   3.267


  14 in total

1.  Rotavirus genotypes P[4]G9, P[6]G9, and P[8]G9 in hospitalized children with acute gastroenteritis in Rio de Janeiro, Brazil.

Authors:  I T Araújo; M S Ferreira; A M Fialho; R M Assis; C M Cruz; M Rocha; J P Leite
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

2.  Serotypes and subgroups of rotavirus isolated from children in central Brazil.

Authors:  D Cardoso das; C M Soares; M S Azevedo; J P Leite; V Munford; M L Rácz
Journal:  J Health Popul Nutr       Date:  2000-06       Impact factor: 2.000

Review 3.  The magnitude of the global problem of diarrhoeal disease: a ten-year update.

Authors:  C Bern; J Martines; I de Zoysa; R I Glass
Journal:  Bull World Health Organ       Date:  1992       Impact factor: 9.408

4.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

5.  Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.

Authors:  B S Coulson; L E Unicomb; G A Pitson; R F Bishop
Journal:  J Clin Microbiol       Date:  1987-03       Impact factor: 5.948

6.  Molecular epidemiology of human group A rotavirus infections in the United Kingdom between 1995 and 1998.

Authors:  M Iturriza-Gómara; J Green; D W Brown; M Ramsay; U Desselberger; J J Gray
Journal:  J Clin Microbiol       Date:  2000-12       Impact factor: 5.948

7.  A combined enzyme immunoassay for rotavirus and adenovirus (EIARA).

Authors:  H G Pereira; R S Azeredo; J P Leite; Z P Andrade; L De Castro
Journal:  J Virol Methods       Date:  1985-01       Impact factor: 2.014

Review 8.  Rotavirus serotypes: classification and importance in epidemiology, immunity, and vaccine development.

Authors:  Y Hoshino; A Z Kapikian
Journal:  J Health Popul Nutr       Date:  2000-06       Impact factor: 2.000

9.  Further characterization of field strains of rotavirus from Nigeria VP4 genotype P6 most frequently identified among symptomatically infected children.

Authors:  M I Adah; A Rohwedder; O D Olaleye; O A Durojaiye; H Werchau
Journal:  J Trop Pediatr       Date:  1997-10       Impact factor: 1.165

10.  Identification of group A rotavirus gene 4 types by polymerase chain reaction.

Authors:  J R Gentsch; R I Glass; P Woods; V Gouvea; M Gorziglia; J Flores; B K Das; M K Bhan
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

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  3 in total

1.  Predominance of rotavirus genotype G9 during the 1999, 2000, and 2002 seasons among hospitalized children in the city of Salvador, Bahia, Brazil: implications for future vaccine strategies.

Authors:  Norma Santos; Eduardo M Volotão; Caroline C Soares; Gúbio S Campos; Silvia Ines Sardi; Yasutaka Hoshino
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

2.  Occurrence of group A rotavirus mixed P genotypes infections in children living in Goiânia-Goiás, Brazil.

Authors:  E R L Freitas; C M A Soares; F S Fiaccadori; M Souza; J A Parente; P S S Costa; D D P Cardoso
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-06-03       Impact factor: 3.267

3.  Surveillance study (2000 to 2001) of G- and P-type human rotaviruses circulating in South Korea.

Authors:  Bok Soon Min; Yoon Ju Noh; Jin Ho Shin; Sun Young Baek; Jae Ok Kim; Kyung Il Min; Seung Rel Ryu; Byoug Guk Kim; Do Keun Kim; Seok Ho Lee; Hong Ki Min; Byung Yoon Ahn; Sue Nie Park
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

  3 in total

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