| Literature DB >> 12882563 |
Cheol-Joo Woo1, Heui-Dong Park.
Abstract
An extracellular chitinase of Bacillus sp. WY22 was purified by 9.6-fold. It had a Mr of 35 kDa, an apparent Km value for colloidal chitin of 3 mg ml(-1) and was optimally active at 37 degrees C and pH 5.5 over 1 h. The enzyme could also hydrolyse swollen chitin, glycol chitin and chitosan with relative activities of 76%, 34% and 23% compared with colloidal chitin. It formed chitotriose as a major product from colloidal chitin and glycol chitin.Entities:
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Year: 2003 PMID: 12882563 DOI: 10.1023/a:1022493226727
Source DB: PubMed Journal: Biotechnol Lett ISSN: 0141-5492 Impact factor: 2.461