OBJECTIVE: To test if subpopulations of chondrocytes from different cartilage zones could be used to engineer cartilage constructs with features of normal stratification. ESIGN: Chondrocytes from the superficial and middle zones of immature bovine cartilage were cultured in alginate, released, and seeded either separately or sequentially to form cartilage constructs. Constructs were cultured for 1 or 2 weeks and were assessed for growth, compressive properties, and deposition, and localization of matrix molecules and superficial zone protein (SZP). RESULTS: The cartilaginous constructs formed from superficial zone chondrocytes exhibited less matrix growth and lower compressive properties than constructs from middle zone chondrocytes, with the stratified superficial-middle constructs exhibiting intermediate properties. Expression of SZP was highest at the construct surfaces, with the localization of SZP in superficial-middle constructs being concentrated at the superficial surface. CONCLUSIONS: Manipulation of subpopulations of chondrocytes can be useful in engineering cartilage tissue with a biomimetic approach, and in fabricating constructs that exhibit stratified features of normal articular cartilage.
OBJECTIVE: To test if subpopulations of chondrocytes from different cartilage zones could be used to engineer cartilage constructs with features of normal stratification. ESIGN: Chondrocytes from the superficial and middle zones of immature bovinecartilage were cultured in alginate, released, and seeded either separately or sequentially to form cartilage constructs. Constructs were cultured for 1 or 2 weeks and were assessed for growth, compressive properties, and deposition, and localization of matrix molecules and superficial zone protein (SZP). RESULTS: The cartilaginous constructs formed from superficial zone chondrocytes exhibited less matrix growth and lower compressive properties than constructs from middle zone chondrocytes, with the stratified superficial-middle constructs exhibiting intermediate properties. Expression of SZP was highest at the construct surfaces, with the localization of SZP in superficial-middle constructs being concentrated at the superficial surface. CONCLUSIONS: Manipulation of subpopulations of chondrocytes can be useful in engineering cartilage tissue with a biomimetic approach, and in fabricating constructs that exhibit stratified features of normal articular cartilage.
Authors: Shawn P Grogan; Chantal Pauli; Peter Chen; Jiang Du; Christine B Chung; Seong Deok Kong; Clifford W Colwell; Martin K Lotz; Sungho Jin; Darryl D D'Lima Journal: Tissue Eng Part C Methods Date: 2012-02-10 Impact factor: 3.056
Authors: Nandana Bhardwaj; Quynhhoa T Nguyen; Albert C Chen; David L Kaplan; Robert L Sah; Subhas C Kundu Journal: Biomaterials Date: 2011-05-20 Impact factor: 12.479
Authors: Katherine M Larson; Ling Zhang; Khaled A Elsaid; Tannin A Schmidt; Braden C Fleming; Gary J Badger; Gregory D Jay Journal: J Orthop Res Date: 2017-03-02 Impact factor: 3.494
Authors: Michael B Albro; Rajan E Banerjee; Roland Li; Sevan R Oungoulian; Bo Chen; Amaya P del Palomar; Clark T Hung; Gerard A Ateshian Journal: J Biomech Date: 2011-04-08 Impact factor: 2.712