OBJECTIVE: To investigate the changes of subgroups of peripheral blood T lymphocytes in patients with severe acute respiratory syndrome (SARS) and its clinical significance. METHODS: Subgroups of blood T lymphocytes in 93 patients with SARS were detected by flow cytometer. The results detected in 64 normal subjects and 50 patients with AIDS served as controls. RESULTS: The numbers of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes all significantly decreased in acute phase of patients with SARS [(722 +/- 533)/microliter, (438 +/- 353)/microliter, (307 +/- 217)/microliter] compared with those in normal controls [(1527 +/- 470)/microliter, (787 +/- 257)/microliter, (633 +/- 280)/microliter, all P <0.01], which was different from what we observed in patients with AIDS who had decreased CD(4)(+) [(296 +/- 298)/microliter] but increased CD(8)(+) [(818 +/- 566)/microliter] counts. The counts of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes decreased more apparently in patients with severe SARS. All the five patients who died had CD(4)(+) counts less than 200/microliter. As the patients' condition improved, CD(3)(+), CD(4)(+), and CD(8)(+) counts gradually returned to normal ranges. CONCLUSION: The damage of cellular immunity is probably an important mechanism of pathogenesis of SARS.
OBJECTIVE: To investigate the changes of subgroups of peripheral blood T lymphocytes in patients with severe acute respiratory syndrome (SARS) and its clinical significance. METHODS: Subgroups of blood T lymphocytes in 93 patients with SARS were detected by flow cytometer. The results detected in 64 normal subjects and 50 patients with AIDS served as controls. RESULTS: The numbers of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes all significantly decreased in acute phase of patients with SARS [(722 +/- 533)/microliter, (438 +/- 353)/microliter, (307 +/- 217)/microliter] compared with those in normal controls [(1527 +/- 470)/microliter, (787 +/- 257)/microliter, (633 +/- 280)/microliter, all P <0.01], which was different from what we observed in patients with AIDS who had decreased CD(4)(+) [(296 +/- 298)/microliter] but increased CD(8)(+) [(818 +/- 566)/microliter] counts. The counts of CD(3)(+), CD(4)(+), and CD(8)(+) lymphocytes decreased more apparently in patients with severe SARS. All the five patients who died had CD(4)(+) counts less than 200/microliter. As the patients' condition improved, CD(3)(+), CD(4)(+), and CD(8)(+) counts gradually returned to normal ranges. CONCLUSION: The damage of cellular immunity is probably an important mechanism of pathogenesis of SARS.
Authors: James V Lawler; Timothy P Endy; Lisa E Hensley; Aura Garrison; Elizabeth A Fritz; May Lesar; Ralph S Baric; David A Kulesh; David A Norwood; Leonard P Wasieloski; Melanie P Ulrich; Tom R Slezak; Elizabeth Vitalis; John W Huggins; Peter B Jahrling; Jason Paragas Journal: PLoS Med Date: 2006-04-18 Impact factor: 11.069
Authors: Ken Yan Ching Chow; Chung Chau Hon; Raymond Kin Hi Hui; Raymond Tsz Yeung Wong; Chi Wai Yip; Fanya Zeng; Frederick Chi Ching Leung Journal: Genomics Proteomics Bioinformatics Date: 2003-11 Impact factor: 7.691
Authors: Fanya Zeng; Ken Yan Ching Chow; Chung Chau Hon; Ka Man Law; Chi Wai Yip; Kwok Hung Chan; Joseph S Malik Peiris; Frederick Chi Ching Leung Journal: Biochem Biophys Res Commun Date: 2004-03-19 Impact factor: 3.575