Literature DB >> 12871716

Synthesis and evaluation of fluorescent probes for the detection of calpain activity.

Stifun Mittoo1, Lars E Sundstrom, Mark Bradley.   

Abstract

Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in alpha-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region.

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Year:  2003        PMID： 12871716     DOI： 10.1016/s0003-2697(03)00324-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

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Journal:  PLoS One       Date:  2011-08-17       Impact factor: 3.240

5.  Falstatin, a cysteine protease inhibitor of Plasmodium falciparum, facilitates erythrocyte invasion.

Authors:  Kailash C Pandey; Naresh Singh; Shirin Arastu-Kapur; Matthew Bogyo; Philip J Rosenthal
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6.  Accumulation of α-synuclein in dementia with Lewy bodies is associated with decline in the α-synuclein-degrading enzymes kallikrein-6 and calpain-1.

Authors:  J Scott Miners; Ruth Renfrew; Marta Swirski; Seth Love
Journal:  Acta Neuropathol Commun       Date:  2014-12-05       Impact factor: 7.801

7.  Conformationally restricted calpain inhibitors.

Authors:  S E Adams; E J Robinson; D J Miller; P J Rizkallah; M B Hallett; R K Allemann
Journal:  Chem Sci       Date:  2015-08-24       Impact factor: 9.825

8.  Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique.

Authors:  Shuai Chen; Li-li Chen; Hai-bin Luo; Tao Sun; Jing Chen; Fei Ye; Jian-hua Cai; Jing-kang Shen; Xu Shen; Hua-liang Jiang
Journal:  Acta Pharmacol Sin       Date:  2005-01       Impact factor: 6.150

9.  Severe acute respiratory syndrome coronavirus 3C-like proteinase N terminus is indispensable for proteolytic activity but not for enzyme dimerization. Biochemical and thermodynamic investigation in conjunction with molecular dynamics simulations.

Authors:  Shuai Chen; Lili Chen; Jinzhi Tan; Jing Chen; Li Du; Tao Sun; Jianhua Shen; Kaixian Chen; Hualiang Jiang; Xu Shen
Journal:  J Biol Chem       Date:  2004-10-26       Impact factor: 5.157
  9 in total

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