SETTING: Tuberculosis Research Centre, Chennai, India. OBJECTIVE: To rapidly identify multidrug-resistant Mycobacterium tuberculosis using phenotypic and genotypic methods. DESIGN: Two genotypic assays, DNA sequencing and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and one phenotypic assay, phage amplified biological assay (PhaB) were standardised in-house and performed on coded 101 rifampicin-resistant and 100 rifampicin-sensitive M. tuberculosis clinical isolates for the identification of rifampicin resistance. RESULTS AND CONCLUSION: The results obtained using the three assays were compared with those from the conventional indirect sensitivity test. The sensitivities and specificities of DNA sequencing, PCR-SSCP and PhaB were 97% and 100%, 76% and 100%, and 97% and 84%, respectively. DNA sequencing was found to be more sensitive and specific than the other tests.
SETTING: Tuberculosis Research Centre, Chennai, India. OBJECTIVE: To rapidly identify multidrug-resistant Mycobacterium tuberculosis using phenotypic and genotypic methods. DESIGN: Two genotypic assays, DNA sequencing and polymerase chain reaction single strand conformation polymorphism (PCR-SSCP), and one phenotypic assay, phage amplified biological assay (PhaB) were standardised in-house and performed on coded 101 rifampicin-resistant and 100 rifampicin-sensitive M. tuberculosis clinical isolates for the identification of rifampicin resistance. RESULTS AND CONCLUSION: The results obtained using the three assays were compared with those from the conventional indirect sensitivity test. The sensitivities and specificities of DNA sequencing, PCR-SSCP and PhaB were 97% and 100%, 76% and 100%, and 97% and 84%, respectively. DNA sequencing was found to be more sensitive and specific than the other tests.
Authors: Wanyuan Ao; Stephen Aldous; Evelyn Woodruff; Brian Hicke; Larry Rea; Barry Kreiswirth; Robert Jenison Journal: J Clin Microbiol Date: 2012-04-18 Impact factor: 5.948
Authors: Danica Helb; Martin Jones; Elizabeth Story; Catharina Boehme; Ellen Wallace; Ken Ho; JoAnn Kop; Michelle R Owens; Richard Rodgers; Padmapriya Banada; Hassan Safi; Robert Blakemore; N T Ngoc Lan; Edward C Jones-López; Michael Levi; Michele Burday; Irene Ayakaka; Roy D Mugerwa; Bill McMillan; Emily Winn-Deen; Lee Christel; Peter Dailey; Mark D Perkins; David H Persing; David Alland Journal: J Clin Microbiol Date: 2009-10-28 Impact factor: 5.948
Authors: Hamidou Traore; Sam Ogwang; Kim Mallard; Moses L Joloba; Francis Mumbowa; Kalpana Narayan; Susan Kayes; Edward C Jones-Lopez; Peter G Smith; Jerrold J Ellner; Roy D Mugerwa; Kathleen D Eisenach; Ruth McNerney Journal: Ann Clin Microbiol Antimicrob Date: 2007-01-09 Impact factor: 3.944