OBJECTIVES: To investigate the role of the inflammatory cytokine interleukin-1beta (IL-1beta) in the regulation of mucin secretion by middle ear epithelia. Study design and setting Primary chinchilla middle ear epithelial cultures were established and exposed to IL-1beta in a dose- and time-dependent manner. Mucin secretion was characterized by exclusion chromatography and liquid scintillation. RESULTS: Epithelial cultures exposed to increasing doses of IL-1beta demonstrated greater amounts of mucin secretion (P = 0.007). Additionally, cultures exposed to IL-1beta at 50 ng/mL showed increased secretion of mucin over control noticeable at 6 hours, but significant at the 15- and 24-hour time points (P <.0001 for each). CONCLUSIONS: IL-1beta upregulates mucin secretion from cultured middle ear epithelial cells in a dose- and time-dependent manner. SIGNIFICANCE: Elucidating the effect of specific cytokines on the regulation of mucin secretion is vital to understanding the pathophysiology of otitis media and the development of novel therapeutic strategies.
OBJECTIVES: To investigate the role of the inflammatory cytokine interleukin-1beta (IL-1beta) in the regulation of mucin secretion by middle ear epithelia. Study design and setting Primary chinchilla middle ear epithelial cultures were established and exposed to IL-1beta in a dose- and time-dependent manner. Mucin secretion was characterized by exclusion chromatography and liquid scintillation. RESULTS: Epithelial cultures exposed to increasing doses of IL-1beta demonstrated greater amounts of mucin secretion (P = 0.007). Additionally, cultures exposed to IL-1beta at 50 ng/mL showed increased secretion of mucin over control noticeable at 6 hours, but significant at the 15- and 24-hour time points (P <.0001 for each). CONCLUSIONS: IL-1beta upregulates mucin secretion from cultured middle ear epithelial cells in a dose- and time-dependent manner. SIGNIFICANCE: Elucidating the effect of specific cytokines on the regulation of mucin secretion is vital to understanding the pathophysiology of otitis media and the development of novel therapeutic strategies.
Authors: Joseph E Kerschner; Geza Erdos; Fen Ze Hu; Amy Burrows; Joseph Cioffi; Pawjai Khampang; Margaret Dahlgren; Jay Hayes; Randy Keefe; Benjamin Janto; J Christopher Post; Garth D Ehrlich Journal: Ann Otol Rhinol Laryngol Date: 2010-04 Impact factor: 1.547
Authors: Tina L Samuels; Justin C Yan; Pawjai Khampang; Peter W Dettmar; Alexander MacKinnon; Wenzhou Hong; Nikki Johnston; Blake C Papsin; Robert H Chun; Michael E McCormick; Joseph E Kerschner Journal: JAMA Otolaryngol Head Neck Surg Date: 2017-08-01 Impact factor: 6.223