Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 An in vivo dual-luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae.

Literature DB >> 12869712

An in vivo dual-luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae.

Abstract

A new in vivo assay system has been developed to study programmed frameshifting in the yeast Saccharomyces cerevisiae. Frameshift signals are inserted between the Renilla and firefly luciferase reporter genes contained in a yeast expression vector and the two activities are directly measured from cell lysates in one tube. Similar to other bicistronic reporter systems, this one allows the efficient estimation of recoding efficiency by comparison of the normalized activity ratios from each luciferase protein. The assay system has been applied to HIV-1 and L-A directed programmed -1 frameshifting and Ty1 and Ty3 directed +1 frameshifting. The assay system is amenable to high-throughput screening.

Entities: Species

Mesh：

Substances：

Year: 2003 PMID： 12869712 PMCID： PMC1236998 DOI： 10.1261/rna.5930803

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

19 in total

1. Nonsense-mediated decay mutants do not affect programmed -1 frameshifting.

Authors: L Bidou; G Stahl; I Hatin; O Namy; J P Rousset; P J Farabaugh
Journal: RNA Date: 2000-07 Impact factor: 4.942

2. The frameshift signal of HIV-1 involves a potential intramolecular triplex RNA structure.

Authors: Jonathan D Dinman; Sara Richter; Ewan P Plant; Ronald C Taylor; Amy B Hammell; Tariq M Rana
Journal: Proc Natl Acad Sci U S A Date: 2002-04-16 Impact factor: 11.205

3. Genetic Control of Biochemical Reactions in Neurospora.

Authors: G W Beadle; E L Tatum
Journal: Proc Natl Acad Sci U S A Date: 1941-11-15 Impact factor: 11.205

Review 4. Recoding: dynamic reprogramming of translation.

Authors: R F Gesteland; J F Atkins
Journal: Annu Rev Biochem Date: 1996 Impact factor: 23.643

5. A dual-luciferase reporter system for studying recoding signals.

Authors: G Grentzmann; J A Ingram; P J Kelly; R F Gesteland; J F Atkins
Journal: RNA Date: 1998-04 Impact factor: 4.942

6. Two chromosomal genes required for killing expression in killer strains of Saccharomyces cerevisiae.

Authors: R B Wickner; M J Leibowitz
Journal: Genetics Date: 1976-03-25 Impact factor: 4.562

Review 7. Nonsense-mediated mRNA decay in Saccharomyces cerevisiae.

Authors: C I González; A Bhattacharya; W Wang; S W Peltz
Journal: Gene Date: 2001-08-22 Impact factor: 3.688

8. A -1 ribosomal frameshift in a double-stranded RNA virus of yeast forms a gag-pol fusion protein.

Authors: J D Dinman; T Icho; R B Wickner
Journal: Proc Natl Acad Sci U S A Date: 1991-01-01 Impact factor: 11.205

9. Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds.

Authors: D Mumberg; R Müller; M Funk
Journal: Gene Date: 1995-04-14 Impact factor: 3.688

Review 10. Translating old drugs into new treatments: ribosomal frameshifting as a target for antiviral agents.

Authors: J D Dinman; M J Ruiz-Echevarria; S W Peltz
Journal: Trends Biotechnol Date: 1998-04 Impact factor: 19.536

100 in total

Review 1. Targeting frameshifting in the human immunodeficiency virus.

Authors: Léa Brakier-Gingras; Johanie Charbonneau; Samuel E Butcher
Journal: Expert Opin Ther Targets Date: 2012-03 Impact factor: 6.902

2. A programmed -1 ribosomal frameshift signal can function as a cis-acting mRNA destabilizing element.

Authors: Ewan P Plant; Pinger Wang; Jonathan L Jacobs; Jonathan D Dinman
Journal: Nucleic Acids Res Date: 2004-02-03 Impact factor: 16.971

3. Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae.

Authors: David S McNabb; Robin Reed; Robert A Marciniak
Journal: Eukaryot Cell Date: 2005-09

4. An arc of unpaired "hinge bases" facilitates information exchange among functional centers of the ribosome.

Authors: Rasa Rakauskaite; Jonathan D Dinman
Journal: Mol Cell Biol Date: 2006-09-25 Impact factor: 4.272

5. Ribosomal protein L3: gatekeeper to the A site.

Authors: Arturas Meskauskas; Jonathan D Dinman
Journal: Mol Cell Date: 2007-03-23 Impact factor: 17.970

6. Communication between RACK1/Asc1 and uS3 (Rps3) is essential for RACK1/Asc1 function in yeast Saccharomyces cerevisiae.

Authors: Nishant Singh; Supriya Jindal; Arnab Ghosh; Anton A Komar
Journal: Gene Date: 2019-05-01 Impact factor: 3.688