PURPOSE: To investigate the antigenicity of porcine corneal stroma as xenograft to man. METHODS: The localization of alpha-gal epitope in the porcine eye was determined using biotinylated Griffonia simplicifolia 1 isolectin B4. Porcine corneal stromal was inserted into corneal stromal pockets of cynomolgus monkeys. Immunohistochemistry was performed to analyze the immunological reaction in the monkey. RESULTS: Immunohistochemistry showed no alpha-gal epitope in the porcine cornea except for several keratocytes in the anterior-most part. Haze and keratic precipitates developed in two corneas out of three corneas that were followed up until 6 months after the surgery. In these two corneas, infiltrating cells included CD4+, CD8+, or HAM56+ cells, suggesting that haze and keratic precipitates were induced by cellular rejection to porcine corneal stroma. CONCLUSIONS: Porcine corneal stroma induces no hyperacute rejection but mild cellular rejection when transplanted in the cornea of cynomolgus monkeys.
PURPOSE: To investigate the antigenicity of porcine corneal stroma as xenograft to man. METHODS: The localization of alpha-gal epitope in the porcine eye was determined using biotinylated Griffonia simplicifolia 1 isolectin B4. Porcine corneal stromal was inserted into corneal stromal pockets of cynomolgus monkeys. Immunohistochemistry was performed to analyze the immunological reaction in the monkey. RESULTS: Immunohistochemistry showed no alpha-gal epitope in the porcine cornea except for several keratocytes in the anterior-most part. Haze and keratic precipitates developed in two corneas out of three corneas that were followed up until 6 months after the surgery. In these two corneas, infiltrating cells included CD4+, CD8+, or HAM56+ cells, suggesting that haze and keratic precipitates were induced by cellular rejection to porcine corneal stroma. CONCLUSIONS: Porcine corneal stroma induces no hyperacute rejection but mild cellular rejection when transplanted in the cornea of cynomolgus monkeys.
Authors: Yi Shao; Yao Yu; Chong-Gang Pei; Qiong Zhou; Qiu-Ping Liu; Gang Tan; Jing-Ming Li; Gui-Ping Gao; Lu Yang Journal: Int J Ophthalmol Date: 2012-08-18 Impact factor: 1.779
Authors: Hidetaka Hara; Naoko Koike; Cassandra Long; Jordan Piluek; Danny S Roh; Nirmala SundarRaj; James L Funderburgh; Yoshiaki Mizuguchi; Kumiko Isse; Carol J Phelps; Suyapa F Ball; David L Ayares; David K C Cooper Journal: Invest Ophthalmol Vis Sci Date: 2011-07-15 Impact factor: 4.799
Authors: Mee Kum Kim; Joo Youn Oh; Jung Hwa Ko; Hyun Ju Lee; Jin Ho Jung; Won Ryang Wee; Jin Hak Lee; Chung-Gyu Park; Sang Joon Kim; Curie Ahn; Seung-Jun Kim; Seung Yong Hwang Journal: J Korean Med Sci Date: 2009-04-20 Impact factor: 2.153
Authors: Hyeon Il Lee; Mee Kum Kim; Joo Youn Oh; Jung Hwa Ko; Hyun Ju Lee; Won Ryang Wee; Jin Hak Lee Journal: J Korean Med Sci Date: 2008-06 Impact factor: 2.153