OBJECTIVE: Epidemiologic data suggest that the malignant transformation of ovarian epithelium may be linked to altered steroid hormone homeostasis. STUDY DESIGN: Estrogen receptor-alpha, estrogen receptor-beta, progesterone receptor A, and progesterone receptor B messenger RNA and protein expression were evaluated by reverse transcriptase-polymerase chain reaction and Western blot analysis in primary cell cultures of human ovarian surface epithelium (n = 23 cultures) and Cedars-Sinai ovarian cancer (n = 23 cultures). RESULTS: The ratio of estrogen receptor-alpha/estrogen receptor-beta messenger RNA expression was 10 times higher in primary ovarian cancer cultures (9.94 +/- 3.90) than in normal ovarian surface epithelium cultures (1.00 +/- 0.16, P =.04). Estrogen receptor-alpha/estrogen receptor-beta protein ratio in primary ovarian cancer cultures (2.13 +/- 0.43) was twice that of normal human ovarian surface epithelium cultures (1.00 +/- 0.13, P =.05). Individual estrogen receptor-alpha and estrogen receptor-beta messenger RNA and protein expression were not significantly different. Progesterone receptor B protein levels in primary ovarian cancer cultures (2.08 +/- 0.42) were twice that of normal surface ovarian epithelium cultures (1.00 +/- 0.10, P =.04), although differences in progesterone receptor B messenger RNA and progesterone receptor A protein expression were not observed. CONCLUSION: Malignant ovarian epithelial cells demonstrated multiple alterations in the expression of sex steroid hormone receptors.
OBJECTIVE: Epidemiologic data suggest that the malignant transformation of ovarian epithelium may be linked to altered steroid hormone homeostasis. STUDY DESIGN:Estrogen receptor-alpha, estrogen receptor-beta, progesterone receptor A, and progesterone receptor B messenger RNA and protein expression were evaluated by reverse transcriptase-polymerase chain reaction and Western blot analysis in primary cell cultures of human ovarian surface epithelium (n = 23 cultures) and Cedars-Sinai ovarian cancer (n = 23 cultures). RESULTS: The ratio of estrogen receptor-alpha/estrogen receptor-beta messenger RNA expression was 10 times higher in primary ovarian cancer cultures (9.94 +/- 3.90) than in normal ovarian surface epithelium cultures (1.00 +/- 0.16, P =.04). Estrogen receptor-alpha/estrogen receptor-beta protein ratio in primary ovarian cancer cultures (2.13 +/- 0.43) was twice that of normal human ovarian surface epithelium cultures (1.00 +/- 0.13, P =.05). Individual estrogen receptor-alpha and estrogen receptor-beta messenger RNA and protein expression were not significantly different. Progesterone receptor B protein levels in primary ovarian cancer cultures (2.08 +/- 0.42) were twice that of normal surface ovarian epithelium cultures (1.00 +/- 0.10, P =.04), although differences in progesterone receptor B messenger RNA and progesterone receptor A protein expression were not observed. CONCLUSION:Malignant ovarian epithelial cells demonstrated multiple alterations in the expression of sex steroid hormone receptors.
Authors: Susan M Dougherty; Williard Mazhawidza; Aimee R Bohn; Krista A Robinson; Kathleen A Mattingly; Kristy A Blankenship; Mary O Huff; William G McGregor; Carolyn M Klinge Journal: Endocr Relat Cancer Date: 2006-03 Impact factor: 5.678
Authors: Galina Lurie; Lynne R Wilkens; Pamela J Thompson; Katharine E McDuffie; Michael E Carney; Keith Y Terada; Marc T Goodman Journal: Cancer Causes Control Date: 2008-08-15 Impact factor: 2.506
Authors: Galina Lurie; Lynne R Wilkens; Pamela J Thompson; Yurii B Shvetsov; Rayna K Matsuno; Michael E Carney; Rachel T Palmieri; Anna H Wu; Malcolm C Pike; Celeste L Pearce; Usha Menon; Aleksandra Gentry-Maharaj; Simon A Gayther; Susan J Ramus; Alice S Whittemore; Valerie McGuire; Weiva Sieh; Paul D P Pharoah; Honglin Song; Jacek Gronwald; Anna Jakubowska; Cezary Cybulski; Jan Lubinski; Joellen M Schildkraut; Andrew Berchuck; Susanne Krüger Kjær; Estrid Høgdall; Peter A Fasching; Matthias W Beckmann; Arif B Ekici; Alexander Hein; Georgia Chenevix-Trench; Penelope M Webb; Jonathan Beesley; Marc T Goodman Journal: PLoS One Date: 2011-06-06 Impact factor: 3.240