| Literature DB >> 12860658 |
Qing Yang1, Wei-Mei Ching, Ju Jiang, Leslie Lousteau, Allen L Richards.
Abstract
The immunodominant 56kDa outer membrane antigens from Orientia tsutsugamushi Kato and Gilliam strains were expressed as inclusion bodies (IBs) in E. coli. The IBs were purified and properly refolded with modifications of a previous procedure used for the production of Karp strain r56 antigen. A mixture of these three r56 proteins exhibited both high sensitivity and specificity for detection of O. tsutsugamushi antibodies by ELISA.Entities:
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Year: 2003 PMID： 12860658 DOI： 10.1111/j.1749-6632.2003.tb07395.x
Source DB: PubMed Journal: Ann N Y Acad Sci ISSN： 0077-8923 Impact factor: 5.691