AIM: To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with human gastric cancer tissue. METHODS: SCID mice were implanted orthotopically with SGC-7901 human gastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 microg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the (3)H TdR infiltration method, the NK cell activity was detected by the LDH release method. RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time. The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by P-selectin Mab. CONCLUSION: P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
AIM: To investigate the effect of cell adhesion molecule P-selectin monoclonal antibody (Mab) on metastasis and immune function of mice orthototopically implanted with humangastric cancer tissue. METHODS: SCID mice were implanted orthotopically with SGC-7901 humangastric carcinoma tissue. Starting from day 3 after operation, animals were given intravenously PBS or P-selectin Mab (100 microg/injection) (for both normal mice and tumor-implanted mice with tumors), twice weekly for 3 weeks. Two animals in each group were sacrificed randomly at the 1st, 2nd, 4th week and 6th week. While T cell and B cell transformation indices were determined with the (3)H TdR infiltration method, the NK cell activity was detected by the LDH release method. RESULTS: The metastatic rate in the P-selectin Mab treated group was lower than that in the PBS treated group (with tumors). The NK activity of normal mice increased over time. The immune functions (T, B cell function, NK activity) of the tumor group in the 6th week were significantly lower than those in the 4th week, but the change was attenuated by P-selectin Mab. CONCLUSION:P-selectin Mab could suppress the metastasis of gastric cancer with no adverse effect on host immune function.
Authors: T Ikonen; M Matikainen; N Mononen; E R Hyytinen; H J Helin; S Tommola; T L Tammela; E Pukkala; J Schleutker; O P Kallioniemi; P A Koivisto Journal: Clin Cancer Res Date: 2001-11 Impact factor: 12.531
Authors: Barbara Muz; Feda Azab; Pilar de la Puente; Scott Rollins; Richard Alvarez; Ziad Kawar; Abdel Kareem Azab Journal: Biomed Res Int Date: 2015-10-11 Impact factor: 3.411