AIM: To establish a rapid, sensitive and specific immunogold assay for detection of hepatitis A virus infection. METHODS: Rabbit monoclonal antibodies to anti-human IgM and IgG (Dako) were dotted on a nitrocellulose membrane (NCM) respectively to capture the human sera IgM and IgG. Then the captured antibodies would conjugate to HAV antigen, which was revealed by mouse anti-HAV IgG conjugated to gold particles. Final results were assessed by blind method. RESULTS: Sera from 96 patients with acute hepatitis were used for our study. Compared with well-recognized standard (Abbott Laboratory, USA), the sensitivity and specificity of IgM-DIGFA (self-made) were 91.3 % (42/46) and 96.0 % (48/50), and those of IgM-ELISA (Kehua, Shanghai) were 97.8 % (45/46) and 100.0 % (50/50). The identical results were produced from the study with reagents at different conditions, and the study was repeated in 15 negative sera and 10 positive sera. The serum anti-HAV IgG was tested with DIGFA at the same time. In comparison with ELISA, the sensitivity and specificity of DIGFA for IgG anti-HAV were 87.2 % (41/47) and 91.8 % (45/49), respectively. CONCLUSION: This assay can detect anti-HAV IgM and IgG simultaneously, and be done within 3 minutes. The simplicity, rapidity and specificity of the assay were useful for screening and epidemiological study.
AIM: To establish a rapid, sensitive and specific immunogold assay for detection of hepatitis A virus infection. METHODS:Rabbit monoclonal antibodies to anti-human IgM and IgG (Dako) were dotted on a nitrocellulose membrane (NCM) respectively to capture the human sera IgM and IgG. Then the captured antibodies would conjugate to HAV antigen, which was revealed by mouse anti-HAV IgG conjugated to gold particles. Final results were assessed by blind method. RESULTS: Sera from 96 patients with acute hepatitis were used for our study. Compared with well-recognized standard (Abbott Laboratory, USA), the sensitivity and specificity of IgM-DIGFA (self-made) were 91.3 % (42/46) and 96.0 % (48/50), and those of IgM-ELISA (Kehua, Shanghai) were 97.8 % (45/46) and 100.0 % (50/50). The identical results were produced from the study with reagents at different conditions, and the study was repeated in 15 negative sera and 10 positive sera. The serum anti-HAV IgG was tested with DIGFA at the same time. In comparison with ELISA, the sensitivity and specificity of DIGFA for IgG anti-HAV were 87.2 % (41/47) and 91.8 % (45/49), respectively. CONCLUSION: This assay can detect anti-HAV IgM and IgG simultaneously, and be done within 3 minutes. The simplicity, rapidity and specificity of the assay were useful for screening and epidemiological study.
Authors: M Renga; G Brandi; G M Paganelli; C Calabrese; S Papa; A Tosti; P Tomassetti; M Miglioli; G Biasco Journal: Gut Date: 1997-09 Impact factor: 23.059
Authors: K H Meyer zum Büschenfelde; A W Lohse; G Gerken; U Treichel; H F Löhr; H Mohr; A Grosse; H P Dienes Journal: J Hepatol Date: 1995 Impact factor: 25.083
Authors: Y Nagayama; K Ohta; M Tsuruta; A Takeshita; H Kimura; K Hamasaki; K Ashizawa; K Nakata; N Yokoyama; S Nagataki Journal: Endocr J Date: 1994-10 Impact factor: 2.349
Authors: G Mazzella; A Salzetta; S Casanova; M C Morelli; N Villanova; R Miniero; S Sottili; V Novelli; A Cipolla; D Festi Journal: Dig Dis Sci Date: 1994-04 Impact factor: 3.199
Authors: Swinburne A J Augustine; Tarsha N Eason; Kaneatra J Simmons; Shannon M Griffin; Clarissa L Curioso; Malini K D Ramudit; Elizabeth A Sams; Kevin H Oshima; Alfred Dufour; Timothy J Wade Journal: J Clin Microbiol Date: 2020-09-22 Impact factor: 5.948