OBJECTIVES: Abnormalities of insulin sensitivity are increasingly reported in HIV infection. Considering that cytokines (particularly TNF-alpha and IL-1beta) can induce insulin resistance in infections, we investigated the relationship between insulin sensitivity and cytokine release from peripheral blood mononuclear cells (PBMCs) in HIV-infected patients. METHODS: Fourteen HIV-positive patients treated with dual-NRTI (nucleosidic reverse transcriptase inhibitors) regimens, and fourteen healthy controls were studied. Insulin resistance was assessed by homeostatic model for insulin resistance (HOMA-IR). Cytokine production by PBMCs ex vivo was measured. RESULTS: Plasma glucose levels did not differ in HIV patients and in controls. Insulin concentrations and HOMA-IR were significantly higher in HIV-infected patients than in controls (respectively, 11.4+/-4.3 vs. 7.86+/-1.1mIU, P=0.005; 2.27+/-0.91 vs. 1.6+/-0.2, P=0.025). A significant positive linear correlation was observed between HOMA-IR and TNF-alpha concentrations in the supernatants of unstimulated PBMC cultures in HIV patients (r=0.771;P=0.001), but not in controls. CONCLUSIONS: Our results are in accordance with previous findings showing that insulin resistance may indeed be present in PI-naive HIV patients, and suggest that either TNF-alpha, or other mediators released in parallel with this cytokine may induce a state of insulin resistance, unrelated to highly active antiviral treatments, in poorly controlled HIV disease.
OBJECTIVES:Abnormalities of insulin sensitivity are increasingly reported in HIV infection. Considering that cytokines (particularly TNF-alpha and IL-1beta) can induce insulin resistance in infections, we investigated the relationship between insulin sensitivity and cytokine release from peripheral blood mononuclear cells (PBMCs) in HIV-infectedpatients. METHODS: Fourteen HIV-positivepatients treated with dual-NRTI (nucleosidic reverse transcriptase inhibitors) regimens, and fourteen healthy controls were studied. Insulin resistance was assessed by homeostatic model for insulin resistance (HOMA-IR). Cytokine production by PBMCs ex vivo was measured. RESULTS: Plasma glucose levels did not differ in HIVpatients and in controls. Insulin concentrations and HOMA-IR were significantly higher in HIV-infectedpatients than in controls (respectively, 11.4+/-4.3 vs. 7.86+/-1.1mIU, P=0.005; 2.27+/-0.91 vs. 1.6+/-0.2, P=0.025). A significant positive linear correlation was observed between HOMA-IR and TNF-alpha concentrations in the supernatants of unstimulated PBMC cultures in HIVpatients (r=0.771;P=0.001), but not in controls. CONCLUSIONS: Our results are in accordance with previous findings showing that insulin resistance may indeed be present in PI-naive HIVpatients, and suggest that either TNF-alpha, or other mediators released in parallel with this cytokine may induce a state of insulin resistance, unrelated to highly active antiviral treatments, in poorly controlled HIV disease.
Authors: L B Mulenga; P Musonda; L Chirwa; M Siwingwa; A Mweemba; S Suwilanji; S Fwoloshi; H Phiri; D Phiri; P L Mulenga; T Chisenga; R Nsakanya; A Shibemba; J Todd; S Nzala; T Kaile; C Kankasa; L Hachaambwa; C Claassen; I Sikazwe; J R Koethe; E Sinkala; D C Heimburger; C W Wester Journal: J Infect Dis Ther Date: 2019-08-16
Authors: Madone Mandina Ndona; Benjamin Longo-Mbenza; Roger Wumba; Barthelemy Tandu Umba; Baudouin Buassa-Bu-Tsumbu; Marcel Mbula Mambimbi; Thaddée Odio Wobin; Simon Mbungu Fuele Journal: Int J Gen Med Date: 2012-11-23
Authors: Cecilia M Shikuma; Dominic C Chow; Louie Mar A Gangcuangco; Guangxiang Zhang; Sheila M Keating; Philip J Norris; Todd B Seto; Nisha Parikh; Kalpana J Kallianpur; Beau K Nakamoto; Lorna S Nagamine; Lishomwa C Ndhlovu; Jason D Barbour Journal: PLoS One Date: 2014-02-27 Impact factor: 3.240