Literature DB >> 12845761

Cloning and expression of rat transforming growth factor beta 1 cDNA in osteoblasts.

Y Liu1, Q Zheng, J Du, H Zeng, X Guo, S Qu.   

Abstract

Rat transforming growth factor beta 1 (rTGF beta 1) cDNA from rat lymphocytes was cloned by RT-PCR and inserted into pcDNA3 to construct an eukaryotic expression vector, which was named pcDNA3-TGF beta 1. The cloned gene was confirmed to code rat TGF beta 1 by restriction enzyme analysis. pcDNA3-TGF beta 1 plasmid was transfected into rat osteoblasts by using liposome-mediated gene transfer technique and the expression of TGF beta 1 was detected by using immunohistochemical staining assay. It was found that the rat TGF beta 1 expression product was obviously detectable in the transfected osteoblasts in 48 h. High expression of TGF beta 1 was obtained in the rat osteoblasts in which the constructed TGF beta 1 expression vector was transfected.

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Year:  2000        PMID： 12845761     DOI： 10.1007/bf02887680

Source DB:  PubMed          Journal:  J Tongji Med Univ        ISSN： 0257-716X


  4 in total

1.  Occurrence and magnitude of pressure waves during Er:YAG laser ablation of atherosclerotic tissue: comparison to XeCl excimer laser ablation.

Authors:  C H Rose; K K Haase; M Wehrmann; K R Karsch
Journal:  Lasers Surg Med       Date:  1996       Impact factor: 4.025

2.  cDNA cloning by PCR of rat transforming growth factor beta-1.

Authors:  S W Qian; P Kondaiah; A B Roberts; M B Sporn
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

3.  Local injection of TGF-beta increases the strength of tibial fractures in the rat.

Authors:  H M Nielsen; T T Andreassen; T Ledet; H Oxlund
Journal:  Acta Orthop Scand       Date:  1994-02

4.  TGF-beta 1 forms functionally normal bone in a segmental sheep tibial diaphyseal defect.

Authors:  J P Moxham; D J Kibblewhite; M Dvorak; B Perey; A F Tencer; A G Bruce; D M Strong
Journal:  J Otolaryngol       Date:  1996-12
  4 in total
  2 in total

1.  Molecular tissue engineering: applications for modulation of mesenchymal stem cells proliferation by transforming growth factor beta 1 gene transfer.

Authors:  X Guo; J Du; Q Zheng; Y Liu; D Duan; Y Wu
Journal:  J Tongji Med Univ       Date:  2001

2.  Expression of transforming growth factor beta 1 in mesenchymal stem cells: potential utility in molecular tissue engineering for osteochondral repair.

Authors:  Xiaodong Guo; Jingyuan Du; Qixin Zheng; Shuhua Yang; Yong Liu; Deyu Duan; Chengqing Yi
Journal:  J Huazhong Univ Sci Technolog Med Sci       Date:  2002
  2 in total

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