Literature DB >> 12845329

Transfection-independent production of alphavirus replicon particles based on poxvirus expression vectors.

Nikos Vasilakis1, Darlene Falvey, Seema S Gangolli, John Coleman, Jacek Kowalski, Stephen A Udem, Timothy J Zamb, Gerald R Kovacs.   

Abstract

This report describes a transfection-independent system for packaging alphavirus replicon vectors using modified vaccinia virus Ankara (MVA) vectors to express all of the RNA components necessary for the production of Venezuelan equine encephalitis (VEE) virus replicon particles (VRP). Infection of mammalian cells with these recombinant MVA vectors resulted in robust expression of VEE structural genes, replication of the alphavirus vector and high titers of VRP. In addition, VRP packaging was achieved in a cell type (fetal rhesus lung) that has been approved for the manufacturing of vaccines destined for human use.

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Year:  2003        PMID： 12845329     DOI： 10.1038/nbt845

Source DB:  PubMed          Journal:  Nat Biotechnol        ISSN： 1087-0156            Impact factor:   54.908


  6 in total

Review 1.  Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond.

Authors:  Veaceslav Boldescu; Mira A M Behnam; Nikos Vasilakis; Christian D Klein
Journal:  Nat Rev Drug Discov       Date:  2017-05-05       Impact factor: 84.694

2.  The Microbial Rosetta Stone Database: a compilation of global and emerging infectious microorganisms and bioterrorist threat agents.

Authors:  David J Ecker; Rangarajan Sampath; Paul Willett; Jacqueline R Wyatt; Vivek Samant; Christian Massire; Thomas A Hall; Kumar Hari; John A McNeil; Cornelia Büchen-Osmond; Bruce Budowle
Journal:  BMC Microbiol       Date:  2005-04-25       Impact factor: 3.605

3.  Combining liver- and blood-stage malaria viral-vectored vaccines: investigating mechanisms of CD8+ T cell interference.

Authors:  Emily K Forbes; Sumi Biswas; Katharine A Collins; Sarah C Gilbert; Adrian V S Hill; Simon J Draper
Journal:  J Immunol       Date:  2011-08-29       Impact factor: 5.422

4.  A vaccinia virus recombinant transcribing an alphavirus replicon and expressing alphavirus structural proteins leads to packaging of alphavirus infectious single cycle particles.

Authors:  Juana M Sánchez-Puig; María M Lorenzo; Rafael Blasco
Journal:  PLoS One       Date:  2013-10-09       Impact factor: 3.240

Review 5.  Modified vaccinia virus Ankara as antigen delivery system: how can we best use its potential?

Authors:  Ingo Drexler; Caroline Staib; Gerd Sutter
Journal:  Curr Opin Biotechnol       Date:  2004-12       Impact factor: 9.740

6.  Facile method for delivering chikungunya viral replicons into mosquitoes and mammalian cells.

Authors:  Hui-Chung Lin; Der-Jiang Chiao; Chang-Chi Lin; Szu-Cheng Kuo
Journal:  Sci Rep       Date:  2021-06-10       Impact factor: 4.379
  6 in total

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