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Literature DB >> 12843091

Evaluation of use of recombinant Em18 and affinity-purified Em18 for serological differentiation of alveolar echinococcosis from cystic echinococcosis and other parasitic infections.

Ning Xiao¹, Wulamu Mamuti, Hiroshi Yamasaki, Yasuhito Sako, Minoru Nakao, Kazuhiro Nakaya, Bruno Gottstein, Peter M Schantz, Marshall W Lightowlers, Philip S Craig, Akira Ito.

Abstract

To further evaluate recombinant Em18 antigen (rEm18) for immunodiagnosis of human alveolar echinococcosis, 208 serum samples were examined by enzyme-linked immunosorbent assay (ELISA). To comparatively assess the results of rEm18-ELISA, ELISA and immunoblot analysis with two affinity-purified native antigens were also performed with 45 selected serum samples. The results indicate that rEm18 is highly useful for serodiagnosis.

Entities: Disease Species

Mesh：

Substances：

Year: 2003 PMID： 12843091 PMCID： PMC165307 DOI： 10.1128/JCM.41.7.3351-3353.2003

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

9 in total

Review 1. Immunodiagnostic and molecular approaches for the detection of taeniid cestode infections.

Authors: Akira Ito; Philip S Craig
Journal: Trends Parasitol Date: 2003-09

2. Immunodiagnosis of alveolar echinococcosis by enzyme-linked immunosorbent assay using a partially purified Em18/16 enriched fraction.

Authors: A Ito; L Ma; M Itoh; S Y Cho; Y Kong; S Y Kang; T Horii; X L Pang; M Okamoto; T Yamashita; M W Lightowlers; X G Wang; Y H Liu
Journal: Clin Diagn Lab Immunol Date: 1997-01

3. Evaluation of an enzyme-linked immunosorbent assay (ELISA) with affinity-purified Em18 and an ELISA with recombinant Em18 for differential diagnosis of alveolar echinococcosis: results of a blind test.

Authors: Akira Ito; Ning Xiao; Martine Liance; Marcello O Sato; Yasuhito Sako; Wulamu Mamuti; Yuji Ishikawa; Minoru Nakao; Hiroshi Yamasaki; Kazuhiro Nakaya; Karine Bardonnet; Solange Bresson-Hadni; Dominique A Vuitton
Journal: J Clin Microbiol Date: 2002-11 Impact factor: 5.948

4. Usefulness of hydatid cyst fluid of Echinococcus granulosus developed in mice with secondary infection for serodiagnosis of cystic Echinococcosis in humans.

Authors: Wulamu Mamuti; Hiroshi Yamasaki; Yasuhito Sako; Kazuhiro Nakaya; Minoru Nakao; Marshall W Lightowlers; Akira Ito
Journal: Clin Diagn Lab Immunol Date: 2002-05

5. An epidemiological and ecological study of human alveolar echinococcosis transmission in south Gansu, China.

Authors: P S Craig; P Giraudoux; D Shi; B Bartholomot; G Barnish; P Delattre; J P Quere; S Harraga; G Bao; Y Wang; F Lu; A Ito; D A Vuitton
Journal: Acta Trop Date: 2000-11-02 Impact factor: 3.112

Review 6. Control of echinococcosis and cysticercosis: a public health challenge to international cooperation in China.

Authors: Akira Ito; Carlo Urbani; Qiu Jiamin; Dominique A Vuitton; Qiu Dongchuan; David D Heath; Philip S Craig; Feng Zheng; Peter M Schantz
Journal: Acta Trop Date: 2003-04 Impact factor: 3.112

7. Alveolar echinococcosis: characterization of diagnostic antigen Em18 and serological evaluation of recombinant Em18.

Authors: Yasuhito Sako; Minoru Nakao; Kazuhiro Nakaya; Hiroshi Yamasaki; Bruno Gottstein; Marshall W Lightowers; Peter M Schantz; Akira Ito
Journal: J Clin Microbiol Date: 2002-08 Impact factor: 5.948

8. Echinococcus multilocularis: molecular and immunochemical characterization of diagnostic antigen II/3-10.

Authors: R Felleisen; B Gottstein
Journal: Parasitology Date: 1993-09 Impact factor: 3.234

9. Comparative analysis of full-length antigen II/3 from Echinococcus multilocularis and E. granulosus.

Authors: R Felleisen; B Gottstein
Journal: Parasitology Date: 1994-08 Impact factor: 3.234

9 in total

15 in total

1. Serological diagnosis of echinococcosis: the diagnostic potential of native antigens.

Authors: A Schweiger; F Grimm; I Tanner; B Müllhaupt; K Bertogg; N Müller; P Deplazes
Journal: Infection Date: 2011-11-11 Impact factor: 3.553

2. Development of an immunochromatographic test to detect antibodies against recombinant Em18 for diagnosis of alveolar echinococcosis.

Authors: Yasuhito Sako; Kenta Fukuda; Yukuharu Kobayashi; Akira Ito
Journal: J Clin Microbiol Date: 2008-11-05 Impact factor: 5.948

3. Serological monitoring of progression of alveolar echinococcosis with multiorgan involvement by use of recombinant Em18.

Authors: Yuji Ishikawa; Yasuhito Sako; Sonoyo Itoh; Takaaki Ohtake; Yutaka Kohgo; Takeo Matsuno; Yoshinobu Ohsaki; Naoyuki Miyokawa; Minoru Nakao; Kazuhiro Nakaya; Akira Ito
Journal: J Clin Microbiol Date: 2009-08-05 Impact factor: 5.948

Review 4. Hepatic echinococcosis: clinical and therapeutic aspects.

Authors: Giuseppe Nunnari; Marilia R Pinzone; Salvatore Gruttadauria; Benedetto M Celesia; Giordano Madeddu; Giulia Malaguarnera; Piero Pavone; Alessandro Cappellani; Bruno Cacopardo
Journal: World J Gastroenterol Date: 2012-04-07 Impact factor: 5.742

5. Widespread co-endemicity of human cystic and alveolar echinococcosis on the eastern Tibetan Plateau, northwest Sichuan/southeast Qinghai, China.

Authors: Tiaoying Li; Xingwang Chen; Ren Zhen; Jiamin Qiu; Dongchuan Qiu; Ning Xiao; Akira Ito; Hu Wang; Patrick Giraudoux; Yasuhito Sako; Minoru Nakao; Philip S Craig
Journal: Acta Trop Date: 2009-11-24 Impact factor: 3.112

6. Specific IgG responses to recombinant antigen B and em18 in cystic and alveolar echinococcosis in china.

Authors: Tiaoying Li; Akira Ito; Xingwang Chen; Yasuhito Sako; Jiamin Qiu; Ning Xiao; Dongchuan Qiu; Minoru Nakao; Tetsuya Yanagida; Philip S Craig
Journal: Clin Vaccine Immunol Date: 2009-12-30

7. Differential diagnosis of cystic and alveolar echinococcosis using an immunochromatographic test based on the detection of specific antibodies.

Authors: Jun-yun Wang; Chun-hua Gao; Dietmar Steverding; Xia Wang; Feng Shi; Yue-tao Yang
Journal: Parasitol Res Date: 2013-08-16 Impact factor: 2.289