OBJECTIVE: To clarify the role of very low density lipoprotein (VLDL) and apolipoprotein E (apoE) in adipogenesis, we studied newly developed hyperlipidemic obese (ob/ob;apoE-/-) mice. Because hydrolysis of VLDL is believed to be the major source of adipogenic free fatty acids, a higher plasma level of VLDL in these mice should exaggerate obesity. METHODS AND RESULTS: When fed a high-fat, high-cholesterol diet, ob/ob;apoE-/- mice did not show increased body weight or an increased amount of adipose tissue in spite of increased plasma VLDL levels, whereas ob/ob mice showed an increased body weight and amount of adipose tissue, suggesting that there is a novel apoE-dependent pathway for adipogenesis. In vitro experiments using bone marrow stromal cells and 3T3-L1 cells confirmed this notion. ApoE-deficient VLDL did not induce adipogenesis, whereas normal VLDL induced adipogenesis in these cells. The incubation of apoE-deficient VLDL with recombinant human apoE restored its adipogenic activity. Tetrahydrolipstatin, a lipoprotein lipase inhibitor, did not affect the adipogenic activity of VLDL, suggesting that hydrolysis of VLDL did not play a major role in its effects. In fact, lipid components of VLDL or free fatty acids induced only partial adipogenesis. CONCLUSIONS: Our findings indicate that VLDL induces adipogenesis in an apoE-dependent manner both in vitro and in vivo.
OBJECTIVE: To clarify the role of very low density lipoprotein (VLDL) and apolipoprotein E (apoE) in adipogenesis, we studied newly developed hyperlipidemic obese (ob/ob;apoE-/-) mice. Because hydrolysis of VLDL is believed to be the major source of adipogenic free fatty acids, a higher plasma level of VLDL in these mice should exaggerate obesity. METHODS AND RESULTS: When fed a high-fat, high-cholesterol diet, ob/ob;apoE-/- mice did not show increased body weight or an increased amount of adipose tissue in spite of increased plasma VLDL levels, whereas ob/ob mice showed an increased body weight and amount of adipose tissue, suggesting that there is a novel apoE-dependent pathway for adipogenesis. In vitro experiments using bone marrow stromal cells and 3T3-L1 cells confirmed this notion. ApoE-deficient VLDL did not induce adipogenesis, whereas normal VLDL induced adipogenesis in these cells. The incubation of apoE-deficient VLDL with recombinant humanapoE restored its adipogenic activity. Tetrahydrolipstatin, a lipoprotein lipase inhibitor, did not affect the adipogenic activity of VLDL, suggesting that hydrolysis of VLDL did not play a major role in its effects. In fact, lipid components of VLDL or free fatty acids induced only partial adipogenesis. CONCLUSIONS: Our findings indicate that VLDL induces adipogenesis in an apoE-dependent manner both in vitro and in vivo.
Authors: Jiali Wang; Xiaoyuan Dai Perrard; Jerry L Perrard; Aparna Mukherjee; Corina Rosales; Yuguo Chen; C Wayne Smith; Henry J Pownall; Christie M Ballantyne; Huaizhu Wu Journal: Atherosclerosis Date: 2012-06-19 Impact factor: 5.162
Authors: Joon T Kim; Mark P Jedrychowski; Wei Wei; Daniel Fernandez; Curt R Fischer; Steven M Banik; Bruce M Spiegelman; Jonathan Z Long Journal: Cell Chem Biol Date: 2020-05-12 Impact factor: 8.116
Authors: Susanna M Hofmann; Diego Perez-Tilve; Todd M Greer; Beth A Coburn; Erin Grant; Joshua E Basford; Matthias H Tschöp; David Y Hui Journal: Diabetes Date: 2007-10-03 Impact factor: 9.461