Literature DB >> 12840889

Non-radioactive hybridization in microwells using enzyme linked immune sorbent assay for detection of RT-PCR-amplified CK19- and CEA-mRNA.

X Zhong1, S Kaul, A Eichler, G Bastert.   

Abstract

PCR enzyme linked immune sorbent assay (ELISA) was developed for detection of RT-PCR-amplified cytokeratin 19 (CK19) mRNA and carcinoembryonic antigen (CEA) mRNA. The non-radioactive hybridization was performed in a streptavidin-coated microwell with digoxigenin-labeled PCR products and with biotin-labeled capture probe. PCR ELISA was proved to be expedient, simple, sensitive and safe for identification of CK19-, CEA-RT-PCR products. These results were proven by sequencing.

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Year:  1999        PMID： 12840889     DOI： 10.1007/bf02887729

Source DB:  PubMed          Journal:  J Tongji Med Univ        ISSN： 0257-716X


  8 in total

Review 1.  Minimal residual epithelial cancer: diagnostic approaches and prognostic relevance.

Authors:  K Pantel; S Braun; B Passlick; G Schlimok
Journal:  Prog Histochem Cytochem       Date:  1996

2.  Low level expression of cytokeratins 8, 18 and 19 in vascular smooth muscle cells of human umbilical cord and in cultured cells derived therefrom, with an analysis of the chromosomal locus containing the cytokeratin 19 gene.

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Journal:  Eur J Cell Biol       Date:  1988-12       Impact factor: 4.492

3.  Embryonic expression of the human 40-kD keratin: evidence from a processed pseudogene sequence.

Authors:  E S Savtchenko; T A Schiff; C K Jiang; I M Freedberg; M Blumenberg
Journal:  Am J Hum Genet       Date:  1988-11       Impact factor: 11.025

4.  Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Authors:  H Schrewe; J Thompson; M Bona; L J Hefta; A Maruya; M Hassauer; J E Shively; S von Kleist; W Zimmermann
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

5.  Sensitive detection of occult breast cancer by the reverse-transcriptase polymerase chain reaction.

Authors:  Y H Datta; P T Adams; W R Drobyski; S P Ethier; V H Terry; M S Roth
Journal:  J Clin Oncol       Date:  1994-03       Impact factor: 44.544

6.  Prognostic significance of micrometastatic tumour cells in bone marrow of colorectal cancer patients.

Authors:  F Lindemann; G Schlimok; P Dirschedl; J Witte; G Riethmüller
Journal:  Lancet       Date:  1992-09-19       Impact factor: 79.321

7.  Specific detection of carcinoembryonic antigen-expressing tumor cells in bone marrow aspirates by polymerase chain reaction.

Authors:  M Gerhard; H Juhl; H Kalthoff; H W Schreiber; C Wagener; M Neumaier
Journal:  J Clin Oncol       Date:  1994-04       Impact factor: 44.544

Review 8.  Molecular cloning and expression of carcinoembryonic antigen gene family members.

Authors:  J A Thompson
Journal:  Tumour Biol       Date:  1995
  8 in total
  1 in total

1.  The application of alkaline phosphatase labeled HBV probe in serum detection.

Authors:  Zhiming Tu; Li-Hua Ke; Guangyuan He
Journal:  Virus Genes       Date:  2004-03       Impact factor: 2.332
  1 in total

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