Literature DB >> 12834966

Some potentialities and drawbacks of contemporary size-exclusion chromatography.

Karel Stulík1, Vera Pacáková, Marie Tichá.   

Abstract

The present state of the chromatographic techniques based on differentiation of solutes according to their molecular sizes is briefly surveyed. Attention is centred on high-performance techniques applied to purification and characterization of natural macromolecules, and on discussion of the chromatographic approaches to the determination of the molecular masses and molecular mass distributions of both natural and synthetic polymers. The basic requirements on the selection of the separation system and the experimental conditions are summarized, demonstrated on a few examples and critically evaluated.

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Year:  2003        PMID: 12834966     DOI: 10.1016/s0165-022x(03)00053-8

Source DB:  PubMed          Journal:  J Biochem Biophys Methods        ISSN: 0165-022X


  13 in total

1.  Analyzing insulin samples by size-exclusion chromatography: a column degradation study.

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Journal:  J Pharm Sci       Date:  2015-01-07       Impact factor: 3.534

2.  Use of Cyclodextrin as a Novel Agent in the SEC-HPLC Mobile Phase to Mitigate the Interactions of Proteins or Peptide or their Impurities with the Residual Silanols of Commercial SEC-HPLC Columns with Improved Separation and Resolution.

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3.  Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain.

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Journal:  J Vis Exp       Date:  2017-12-12       Impact factor: 1.355

Review 4.  Characterization and Analysis of Food-Sourced Carbohydrates.

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Journal:  Methods Mol Biol       Date:  2022

5.  Methods to Analyze the Redox Reactivity of Plant Proteins.

Authors:  Thualfeqar Al-Mohanna; George V Popescu; Sorina C Popescu
Journal:  Methods Mol Biol       Date:  2022

Review 6.  Preparative purification of recombinant proteins: current status and future trends.

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Journal:  Biomed Res Int       Date:  2013-12-17       Impact factor: 3.411

7.  Efficient single step chromatographic purification of recombinant human antithrombin (rhAT) from Saccharomyces cerevisiae.

Authors:  Maheswara Reddy Mallu; Sandeep Vemula; Srinivasa Reddy Ronda
Journal:  3 Biotech       Date:  2016-05-17       Impact factor: 2.406

8.  Solution structures and biophysical analysis of full-length group A PAKs reveal they are monomeric and auto-inhibited in cis.

Authors:  Fiona J Sorrell; Lena Marie Kilian; Jonathan M Elkins
Journal:  Biochem J       Date:  2019-04-04       Impact factor: 3.857

9.  Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and their Aggregates.

Authors:  Paula Hong; Stephan Koza; Edouard S P Bouvier
Journal:  J Liq Chromatogr Relat Technol       Date:  2012-11-30       Impact factor: 1.467

10.  Characterization of a Polyethylene Glycol-Amphotericin B Conjugate Loaded with Free AMB for Improved Antifungal Efficacy.

Authors:  Tessa Rui Min Tan; Kong Meng Hoi; Peiqing Zhang; Say Kong Ng
Journal:  PLoS One       Date:  2016-03-23       Impact factor: 3.240

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