Allen A Smith1, Timothy M Silver. 1. School of Graduate Medical Sciences, Barry University, Miami Shores, FL, USA. asmith@mail.barry.edu
Abstract
BACKGROUND: Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials. METHODS: We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl hematoxylin. RESULTS: Each staining procedure stained the same cells. CONCLUSIONS: Zirconyl hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.
BACKGROUND: Despite its occasional unavailability, alcian blue has long been the stain of choice for the pathognomonic mucin-secreting cells of extramammary Paget's disease. Zirconyl hematoxylin is a recently developed substitute for alcian blue, which is made from common laboratory materials. METHODS: We have stained sections from three different areas of the same case of extramammary Paget's disease with zirconyl hematoxylin, destained in 7% HCl in ethanol, restained with alcian blue at pH 2.5, destained in 2.5% trifluoroacetic acid in dichloromethane, and restained with zirconyl hematoxylin. RESULTS: Each staining procedure stained the same cells. CONCLUSIONS:Zirconyl hematoxylin is a useful alternative to alcian blue in the diagnosis of extramammary Paget's disease.
Authors: Tor W Chiu; Pauline S Y Wong; Kawser Ahmed; Stephanie C K Lam; Shun Y Ying; Andrew Burd Journal: World J Surg Date: 2007-10 Impact factor: 3.282