Direct enantiomeric resolution of (+/-)-atenolol, (+/-)-metoprolol, and (+/-)-propranolol by impregnated TLC using L-aspartic acid as chiral selector.

Resolution of three commonly used beta-blockers, (+/-)-atenolol, (+/-)-metoprolol and (+/-)-propranolol, into their enantiomers has been achieved using normal-phase TLC on silica gel plates impregnated with L-aspartic acid as the chiral selector. Different combinations of acetonitrile-methanol-water as mobile phase were found to be successful in resolving the enantiomers. The spots were detected with iodine and the detection limits were found to be 0.26 microg for atenolol and 0.23 microg for each of metoprolol and propranolol as racemate. Copyright 2003 John Wiley & Sons, Ltd.