BACKGROUND: Traditional diagnostic tests, ie, smear, culture, and histopathology of a skin biopsy specimen, are not always conclusive in patients with a clinical diagnosis of cutaneous leishmaniasis (CL). OBJECTIVE: Our purpose was to find out if a polymerase chain reaction (PCR) specific for Leishmania organisms might be more sensitive than the traditional diagnostic techniques, thereby decreasing the number of false-negative diagnoses. METHODS: In a prospective study, smear, culture, and histopathology of skin biopsy specimens from 46 patients with a possible diagnosis of CL were compared with PCR specific for Leishmania. In addition, the Montenegro test as a measure of cellular immunity against the Leishmania parasite was performed. Proven CL was defined as a case in which at least 1 of the 3 traditional tests showed the presence of Leishmania parasites. RESULTS: Of our 46 patients, 22 had leishmaniasis. Of the traditional tests, culture was the most sensitive but there were no statistically significant differences between the sensitivities of the various tests. PCR results were positive in all cases of proven leishmaniasis. Moreover, 3 patients with the clinical diagnosis of CL and negative findings on traditional tests had positive PCR results. Only 1 patient with a strong clinical suggestion of CL and positive Montenegro test results had negative PCR findings; this patient also had negative smear, culture, and histopathology results. CONCLUSION: PCR appears to be the most sensitive single diagnostic test for CL.
BACKGROUND: Traditional diagnostic tests, ie, smear, culture, and histopathology of a skin biopsy specimen, are not always conclusive in patients with a clinical diagnosis of cutaneous leishmaniasis (CL). OBJECTIVE: Our purpose was to find out if a polymerase chain reaction (PCR) specific for Leishmania organisms might be more sensitive than the traditional diagnostic techniques, thereby decreasing the number of false-negative diagnoses. METHODS: In a prospective study, smear, culture, and histopathology of skin biopsy specimens from 46 patients with a possible diagnosis of CL were compared with PCR specific for Leishmania. In addition, the Montenegro test as a measure of cellular immunity against the Leishmania parasite was performed. Proven CL was defined as a case in which at least 1 of the 3 traditional tests showed the presence of Leishmania parasites. RESULTS: Of our 46 patients, 22 had leishmaniasis. Of the traditional tests, culture was the most sensitive but there were no statistically significant differences between the sensitivities of the various tests. PCR results were positive in all cases of proven leishmaniasis. Moreover, 3 patients with the clinical diagnosis of CL and negative findings on traditional tests had positive PCR results. Only 1 patient with a strong clinical suggestion of CL and positive Montenegro test results had negative PCR findings; this patient also had negative smear, culture, and histopathology results. CONCLUSION: PCR appears to be the most sensitive single diagnostic test for CL.
Authors: Andrea K Boggild; Ana Pilar Ramos; Braulio Mark Valencia; Nicolas Veland; Flor Calderon; Jorge Arevalo; Donald E Low; Alejandro Llanos-Cuentas Journal: J Clin Microbiol Date: 2010-12-22 Impact factor: 5.948
Authors: Wendy F van der Meide; Gerard J Schoone; William R Faber; Jim E Zeegelaar; Henry J C de Vries; Yusuf Ozbel; Rudy F M Lai A Fat; Leíla I A R C Coelho; Masoom Kassi; Henk D F H Schallig Journal: J Clin Microbiol Date: 2005-11 Impact factor: 5.948
Authors: Emily R Adams; Gerard J Schoone; Al Farazdag Ageed; Sayda El Safi; Henk D F H Schallig Journal: Am J Trop Med Hyg Date: 2010-04 Impact factor: 2.345
Authors: Andrea K Boggild; Cesar Miranda-Verastegui; Diego Espinosa; Jorge Arevalo; Vanessa Adaui; Gianfranco Tulliano; Alejandro Llanos-Cuentas; Donald E Low Journal: J Clin Microbiol Date: 2007-09-19 Impact factor: 5.948