BACKGROUND: Tissue engineering of heart valves should avoid the disadvantages of conventional prostheses. In this study we tested different decellularization procedures for their potential of cell removal and their ability to preserve the matrix. METHODS: Specimens of porcine aortic and pulmonary roots were treated with either trypsin or sodium-dodecyl-sulfate (SDS) or Triton-X 100 and sodium-deoxycholate with a range of concentrations. Tissue samples were then processed for scanning electron microscopy and laser scanning microscopy. RESULTS: Trypsin achieved only incomplete decellularization and caused severe structural alterations of the matrix. In contrast SDS removed cells completely but caused strong structural alterations. Treatment with Triton-X100 and sodium-deoxycholate achieved both complete decellularization and preservation of the matrix structure. CONCLUSION: Techniques of decellularization are highly variable in efficiency and matrix preservation and was best achieved in our study with Triton-X100 and sodium deoxycholate.
BACKGROUND: Tissue engineering of heart valves should avoid the disadvantages of conventional prostheses. In this study we tested different decellularization procedures for their potential of cell removal and their ability to preserve the matrix. METHODS: Specimens of porcine aortic and pulmonary roots were treated with either trypsin or sodium-dodecyl-sulfate (SDS) or Triton-X 100 and sodium-deoxycholate with a range of concentrations. Tissue samples were then processed for scanning electron microscopy and laser scanning microscopy. RESULTS: Trypsin achieved only incomplete decellularization and caused severe structural alterations of the matrix. In contrast SDS removed cells completely but caused strong structural alterations. Treatment with Triton-X100 and sodium-deoxycholate achieved both complete decellularization and preservation of the matrix structure. CONCLUSION: Techniques of decellularization are highly variable in efficiency and matrix preservation and was best achieved in our study with Triton-X100 and sodium deoxycholate.
Authors: Benedikt Weber; Maximilian Y Emmert; Roman Schoenauer; Chad Brokopp; Laura Baumgartner; Simon P Hoerstrup Journal: Semin Immunopathol Date: 2011-01-29 Impact factor: 9.623
Authors: Petra E Dijkman; Emanuela S Fioretta; Laura Frese; Francesco S Pasqualini; Simon P Hoerstrup Journal: Transfus Med Hemother Date: 2016-07-26 Impact factor: 3.747
Authors: Daniel A Cuzzone; Nicholas J Albano; Seth Z Aschen; Swapna Ghanta; Babak J Mehrara Journal: Lymphat Res Biol Date: 2014-08-21 Impact factor: 2.589
Authors: Leslie Neil Sierad; Agneta Simionescu; Christopher Albers; Joseph Chen; Jordan Maivelett; Mary Elizabeth Tedder; Jun Liao; Dan T Simionescu Journal: Cardiovasc Eng Technol Date: 2010-06 Impact factor: 2.495
Authors: D M Faulk; C A Carruthers; H J Warner; C R Kramer; J E Reing; L Zhang; A D'Amore; S F Badylak Journal: Acta Biomater Date: 2013-09-18 Impact factor: 8.947
Authors: Leigh G Griffiths; Leila Choe; Kelvin H Lee; Kenneth F Reardon; E Christopher Orton Journal: Electrophoresis Date: 2008-11 Impact factor: 3.535