PURPOSE: To determine if genetic ablation of the chemokine receptor CCR2 (involved in leukocyte and endothelial chemotaxis) inhibits the development of corneal neovascularization. METHODS: Wild-type C57BL/6J mice, as well as species-specific counterparts with targeted homozygous disruption of the CCR2, underwent chemical and mechanical denudation of corneal and limbal epithelium. Corneas were harvested 2 weeks after injury. Neovascularization was quantified by CD31 immunostaining. RESULTS: The mean percentages of neovascularized corneal area in control mice and CCR2-deficient mice 2 weeks after denudation were 58.3% and 38.8% (P = 0.047), respectively. CONCLUSIONS: Development of corneal neovascularization is inhibited in CCR2-deficient mice.
PURPOSE: To determine if genetic ablation of the chemokine receptor CCR2 (involved in leukocyte and endothelial chemotaxis) inhibits the development of corneal neovascularization. METHODS: Wild-type C57BL/6J mice, as well as species-specific counterparts with targeted homozygous disruption of the CCR2, underwent chemical and mechanical denudation of corneal and limbal epithelium. Corneas were harvested 2 weeks after injury. Neovascularization was quantified by CD31 immunostaining. RESULTS: The mean percentages of neovascularized corneal area in control mice and CCR2-deficientmice 2 weeks after denudation were 58.3% and 38.8% (P = 0.047), respectively. CONCLUSIONS: Development of corneal neovascularization is inhibited in CCR2-deficientmice.
Authors: Balamurali K Ambati; Miho Nozaki; Nirbhai Singh; Atsunobu Takeda; Pooja D Jani; Tushar Suthar; Romulo J C Albuquerque; Elizabeth Richter; Eiji Sakurai; Michael T Newcomb; Mark E Kleinman; Ruth B Caldwell; Qing Lin; Yuichiro Ogura; Angela Orecchia; Don A Samuelson; Dalen W Agnew; Judy St Leger; W Richard Green; Parameshwar J Mahasreshti; David T Curiel; Donna Kwan; Helene Marsh; Sakae Ikeda; Lucy J Leiper; J Martin Collinson; Sasha Bogdanovich; Tejvir S Khurana; Masabumi Shibuya; Megan E Baldwin; Napoleone Ferrara; Hans-Peter Gerber; Sandro De Falco; Jassir Witta; Judit Z Baffi; Brian J Raisler; Jayakrishna Ambati Journal: Nature Date: 2006-10-18 Impact factor: 49.962
Authors: Surekha Maddula; Don K Davis; Soumya Maddula; Michael K Burrow; Balamurali K Ambati Journal: Ophthalmology Date: 2011-03 Impact factor: 12.079
Authors: T Oshima; K-H Sonoda; C Tsutsumi-Miyahara; H Qiao; T Hisatomi; S Nakao; S Hamano; K Egashira; I F Charo; T Ishibashi Journal: Br J Ophthalmol Date: 2006-02 Impact factor: 4.638