Literature DB >> 12826060

Sequences of versatile broad-host-range vectors of the RK2 family.

Heather N Scott1, Philip D Laible, Deborah K Hanson.   

Abstract

Plasmid pRK404-a smaller derivative of RK2-is a tetracycline-resistant broad-host-range vector that carries a multiple cloning site and the lacZ(alpha) peptide that enables blue/white selection for cloned inserts in Escherichia coli. We present herein the complete and annotated sequence of pRK404 and three related vectors-pRK437, pRK442, and pRK442(H). These derivatives have proven to be valuable tools for genetic manipulation in Gram-negative bacteria. The knowledge of their complete sequences will facilitate efficient future engineering of them and will enhance their general applicability to the design of genetic systems for use in organisms for which new genomic sequence data are becoming available.

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Year:  2003        PMID： 12826060     DOI： 10.1016/s0147-619x(03)00030-1

Source DB:  PubMed          Journal:  Plasmid        ISSN： 0147-619X            Impact factor:   3.466


  16 in total

1.  Towards higher-throughput membrane protein production for structural genomics initiatives.

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Journal:  J Bacteriol       Date:  2013-08-09       Impact factor: 3.490

3.  Transcriptional interference and repression modulate the conjugative ability of the symbiotic plasmid of Rhizobium etli.

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Journal:  J Bacteriol       Date:  2008-04-18       Impact factor: 3.490

4.  Roles of RseB, sigmaE, and DegP in virulence and phase variation of colony morphotype of Vibrio vulnificus.

Authors:  Roslyn N Brown; Paul A Gulig
Journal:  Infect Immun       Date:  2009-06-29       Impact factor: 3.441

5.  Incorporation of selenomethionine into induced intracytoplasmic membrane proteins of Rhodobacter species.

Authors:  Philip D Laible; Aaron N Hata; Adam E Crawford; Deborah K Hanson
Journal:  J Struct Funct Genomics       Date:  2005

6.  Identification of a new gene required for the biosynthesis of rhodoquinone in Rhodospirillum rubrum.

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Journal:  J Bacteriol       Date:  2011-12-22       Impact factor: 3.490

7.  The selection of recombinant binary plasmids generated by Gateway(®) LR cloning in the Escherichia coli strain C2110.

Authors:  Shuchi Wu; Bingyu Zhao
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695

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Authors:  Pongdet Piromyou; Teerana Greetatorn; Kamonluck Teamtisong; Takashi Okubo; Ryo Shinoda; Achara Nuntakij; Panlada Tittabutr; Nantakorn Boonkerd; Kiwamu Minamisawa; Neung Teaumroong
Journal:  Appl Environ Microbiol       Date:  2015-02-20       Impact factor: 4.792

9.  Regulation of fatty acid metabolism by FadR is essential for Vibrio vulnificus to cause infection of mice.

Authors:  Roslyn N Brown; Paul A Gulig
Journal:  J Bacteriol       Date:  2008-10-03       Impact factor: 3.490

10.  USER friendly cloning coupled with chitin-based natural transformation enables rapid mutagenesis of Vibrio vulnificus.

Authors:  Paul A Gulig; Matthew S Tucker; Patrick C Thiaville; Jennifer L Joseph; Roslyn N Brown
Journal:  Appl Environ Microbiol       Date:  2009-06-05       Impact factor: 4.792
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