Literature DB >> 12823841

A method for extraction and analysis of high quality genomic DNA from ixodid ticks.

C A Hill1, J A Gutierrez.   

Abstract

Currently, no published methods describe the extraction of high molecular weight genomic DNA from ixodid ticks (Acari: Ixodidae) and commonly used methods of extraction are not well adapted for use with members of this family. A method for extraction of minimally degraded genomic DNA from ixodid ticks that can be completed in one or two days is described. The method produces DNA which is of sufficient size (>24 kb) for use in Southern analysis and which is readily digestible by restriction endonucleases. Southern analysis using a cytochrome P450 gene probe, demonstrates the success of our method with genomic DNA extracted from two species of Ixodidae, the lone star tick, Amblyomma americanum (Linnaeus) and the cattle fever tick, Boophilus microplus (Canestrini).

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Year:  2003        PMID： 12823841     DOI： 10.1046/j.1365-2915.2003.00425.x

Source DB:  PubMed          Journal:  Med Vet Entomol        ISSN： 0269-283X            Impact factor:   2.739


  9 in total

1.  Comparison of preservation methods of Rhipicephalus appendiculatus (Acari: Ixodidae) for reliable DNA amplification by PCR.

Authors:  Jupiter Mtambo; Wim Van Bortel; Maxime Madder; Patricia Roelants; Thierry Backeljau
Journal:  Exp Appl Acarol       Date:  2006       Impact factor: 2.132

2.  Extraction of total nucleic acids from ticks for the detection of bacterial and viral pathogens.

Authors:  Chris D Crowder; Megan A Rounds; Curtis A Phillipson; John M Picuri; Heather E Matthews; Justina Halverson; Steven E Schutzer; David J Ecker; Mark W Eshoo
Journal:  J Med Entomol       Date:  2010-01       Impact factor: 2.278

3.  Multiple transcripts encode glucose 6-phosphate dehydrogenase in the southern cattle tick, Rhipicephalus (Boophilus) microplus.

Authors:  Pia Untalan Olafson; Kevin B Temeyer; John H Pruett
Journal:  Exp Appl Acarol       Date:  2010-08-15       Impact factor: 2.132

4.  Two simple techniques for the safe Sarcoptes collection and individual mite DNA extraction.

Authors:  Dominga Soglia; Luisa Rambozzi; Sandra Maione; Veronica Spalenza; Stefano Sartore; Samer Alasaad; Paola Sacchi; Luca Rossi
Journal:  Parasitol Res       Date:  2009-08-05       Impact factor: 2.289

5.  A simple and rapid method for DNA isolation from xylophagous insects.

Authors:  Nancy Calderón-Cortés; Mauricio Quesada; Horacio Cano-Camacho; Guadalupe Zavala-Páramo
Journal:  Int J Mol Sci       Date:  2010-12-07       Impact factor: 5.923

6.  To beat or not to beat a tick: comparison of DNA extraction methods for ticks (Ixodes scapularis).

Authors:  Alyssa D Ammazzalorso; Christine P Zolnik; Thomas J Daniels; Sergios-Orestis Kolokotronis
Journal:  PeerJ       Date:  2015-08-13       Impact factor: 2.984

7.  Integration of Ixodes ricinus genome sequencing with transcriptome and proteome annotation of the naïve midgut.

Authors:  Wibke J Cramaro; Dominique Revets; Oliver E Hunewald; Regina Sinner; Anna L Reye; Claude P Muller
Journal:  BMC Genomics       Date:  2015-10-28       Impact factor: 3.969

8.  Reptile-associated Borrelia species in the goanna tick (Bothriocroton undatum) from Sydney, Australia.

Authors:  Jessica L Panetta; Radek Šíma; Nichola E D Calvani; Ondřej Hajdušek; Shona Chandra; Jessica Panuccio; Jan Šlapeta
Journal:  Parasit Vectors       Date:  2017-12-20       Impact factor: 3.876

9.  Combining Citizen Science and Genomics to Investigate Tick, Pathogen, and Commensal Microbiome at Single-Tick Resolution.

Authors:  Gaurav Chauhan; Jesse McClure; Jessica Hekman; Patrick W Marsh; Jeffrey A Bailey; Rachel F Daniels; Diane P Genereux; Elinor K Karlsson
Journal:  Front Genet       Date:  2020-01-21       Impact factor: 4.599
  9 in total

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