AIMS: Angiogenesis is an important factor in tumour growth and metastasis. Vascular endothelial growth factor receptor 2 (VEGFR-2) or KDR plays a crucial role in angiogenesis. The aim of this study was to raise and characterize antibodies against phosphorylated KDR which could be used for studies on human tissues to assess KDR activation and novel inhibitors of KDR activation in clinical trials. METHODS AND RESULTS: Three monoclonal antibodies and one rabbit polyclonal antiserum were produced. The specificity of the antibodies was confirmed by ELISA. One of the mouse antibodies and the rabbit polyclonal antiserum reacted with a 200-kDa band on a Western blot of human umbilical vein endothelial cell (HUVEC) lysates, the molecular weight of KDR. Immunohistochemical staining showed that phosphorylated KDR is present in a wide variety of normal tissues including liver, colon and placenta, and is not restricted to endothelium. It was also present in a number of human tumours including breast carcinomas, colonic carcinomas and non-Hodgkin's lymphomas. The pattern of staining was membranous, cytoplasmic and nuclear. CONCLUSIONS: This study has shown that phosphorylated KDR is present in a wide variety of tumour and tissue types and is not confined to endothelium.
AIMS: Angiogenesis is an important factor in tumour growth and metastasis. Vascular endothelial growth factor receptor 2 (VEGFR-2) or KDR plays a crucial role in angiogenesis. The aim of this study was to raise and characterize antibodies against phosphorylated KDR which could be used for studies on human tissues to assess KDR activation and novel inhibitors of KDR activation in clinical trials. METHODS AND RESULTS: Three monoclonal antibodies and one rabbit polyclonal antiserum were produced. The specificity of the antibodies was confirmed by ELISA. One of the mouse antibodies and the rabbit polyclonal antiserum reacted with a 200-kDa band on a Western blot of human umbilical vein endothelial cell (HUVEC) lysates, the molecular weight of KDR. Immunohistochemical staining showed that phosphorylated KDR is present in a wide variety of normal tissues including liver, colon and placenta, and is not restricted to endothelium. It was also present in a number of humantumours including breast carcinomas, colonic carcinomas and non-Hodgkin's lymphomas. The pattern of staining was membranous, cytoplasmic and nuclear. CONCLUSIONS: This study has shown that phosphorylated KDR is present in a wide variety of tumour and tissue types and is not confined to endothelium.
Authors: Qiuyang Zhang; Chunhua Yu; Sui Peng; Hao Xu; Ellen Wright; Xi Zhang; Xiaofang Huo; Edaire Cheng; Thai H Pham; Kiyotaka Asanuma; Kimmo J Hatanpaa; Davood Rezai; David H Wang; Venetia Sarode; Shelby Melton; Robert M Genta; Stuart J Spechler; Rhonda F Souza Journal: Gastroenterology Date: 2013-10-09 Impact factor: 22.682
Authors: Inês Domingues; José Rino; Jeroen A A Demmers; Primal de Lanerolle; Susana Constantino Rosa Santos Journal: PLoS One Date: 2011-09-28 Impact factor: 3.240
Authors: Alexandra Giatromanolaki; Efthimios Sivridis; Nikolaos E Bechrakis; Gregor Willerding; Georgios St Charitoudis; Michael H Foerster; Kevin C Gatter; Adrian L Harris; Michael I Koukourakis Journal: Clin Exp Metastasis Date: 2011-10-09 Impact factor: 5.150
Authors: Adhemar Longatto-Filho; Céline Pinheiro; Olga Martinho; Marise A R Moreira; Luiz F J Ribeiro; Geraldo S Queiroz; Fernando C Schmitt; Fátima Baltazar; Rui M Reis Journal: BMC Cancer Date: 2009-06-29 Impact factor: 4.430
Authors: Isabelle M Medana; Nicholas P J Day; Rachel Roberts; Navakanit Sachanonta; Helen Turley; Emsri Pongponratn; Tran Tinh Hien; Nicholas J White; Gareth D H Turner Journal: Histopathology Date: 2010-08 Impact factor: 5.087